Cloning And Expression Of Hepatitis B Virus DNA Polymerase Trans-activated HBVDNAPTP1, A Human Novel Gene, And Its Partial Functions

The persistence infection of Hepatitis B virus (HBV) is still a global health problem. Acute infection with HBV may result in chronic infection, which is a leading cause of cirrhosis and hepatocellular carcinoma. However, there is no effectively therapic technique and means for HBV infection at present. Insights into the viral hepatitis pathogenesis of HBV should be sure to obtain an exciting advance in its treatment. When HBV enters the hepatocytes, the molecular mechanism responsible for HBV DNA duplication and expression, immune escape of HBV, chronic infection, hepatic fibrosis, malignant transformation caused by HBV infection are strongly associated with the interaction between virus proteins and hapetocellular proteins. In recent years, the HBV pathogenetic mechanism was researched deeply. The complicated trans-regulation mechanism involved in the course of HBV interaction with hepatocytes. Hepatitis virus protein could influence the gene expression level in the hepatocytes, which related possibly with HBV pathogenesis mechanism. The investigation on the protein-protein interaction with each antigen of HBV in the hepatocytes can further explain the HBV infection mechanism and the achievements will conduce to seek valid means for curing the disease. The study on cloning and expression of many function-unknown genes screeined and their functions is an important source of creative intelligence in viral hepatitis pathogenesis research and the important content of human genome and postgenome project.The recombinant expression plasmids pcDNA3.1(-) with three functional fragments of HBV DNA polymerase were constructed, and were transfected into HepG2 cells.