Preclinical Trial Studies Of Transgenic And Knock-in Prostate Cancer Mouse Models And The Preparation Of Tetramerizing Single Chain Fv Antibody Gene Specific For Human γ-seminoprotein

Objective: Prostate cancer (CaP) is relatively unique to man. There is no naturally occurring prostate cancer in the mouse. Pre-clinical studies stipulate the establishment of a genetically engineered (GE) mouse CaP model with features close to the human situation. A new knock-in mouse adeno-carcinoma prostate model (KIMAP) at a locus of PSP94 (prostate secretory protein of 94 amino acids) gene and the PSP94 gene directed transgenic mouse adenocarcinoma prostate (TGMAP) model were established recently and the KIMAP showed a close-to-human kinetics of tumor development. This study used a new mouse histological grading system similar to the human Gleason grading system, flow cytometry DNA analysis and cDNA microarray analysis to measure and compare the new KIMAP model with human CaP and TGMAP model. This article reports the first use of three-dimensional ultrasound microimaging for measuring tumor progression in the TGMAP model. In this study, we established a mouse serum tumor...