Experimental Study On EPCs And MSCs As Seeded Cells Of Tissue Engineering Heart Valve

Objectives:The ideal valve substitutes should have biological structures of native valves, can adapt to growing, have auto-repairing potential, life-long durability and no immunological response. Tissue engineering heart valves (TEHVs) constructed from autologous living cells theoretically have the potential to meet the characteristics of an ideal valve substitute, because they have the ability of self-renew, remodel, and thereby should have the best hemodynamic and biomechanical properties, as well as longer durability without the limitation of lifelong anticoagulation. To obtain ideal seed cells is the key point of study on TEHVs. So to find a good way to obtain efficiency seed cells are the core problem in TEHV study. In this study, we separate EPCs and MSCs from bone marrow, cells were cultured, multipliced, differentiated and were seeded onto the decelluarized matrices. The condition and possibility of EPCs combined MSCs on TEHVs were discussed in order to obtain ideal seed cells. Methods:1.Immunity magnetism bean and attachement-changing culture medium...