Study On Cloning, Expression And Function Of ADAM28 Gene In Tooth Germ Development

Teeth develop as a result of the interactions between ectoderm epithelia and mesenchyme from neural crest. Gene regulation with spatiotemporal changes play an important role in the course of tooth germ development such as regulating cell proliferation, differentiation and apoptosis and determine the developmental process of tooth germ. ADAM28 is a newly discovered member of a disintegrin and metalloproteinase (ADAM) family, and it is also a secretion type glycoprotein located in cellular membrane with autocatalytic activity. ADAM28, which is considered as a gene related to tooth and tooth root growth, is screened by improved differential hybridization from differential expression genes of children suffering from congenital hypoplasia of tooth root. ADAM28 plays an important role in various physiological processes such as cell proliferation, differentiation, extracellular matrix reconstruction, vasiformation and cell migration. However, there has not been reported about the relationship between ADAM28 and tooth development.Based on the transcription, translation and protein levels, this study is aimed to investigate the spatiotemporal distribution features of ADAM28 during tooth germ development and its effects on the proliferation, differentiation and apoptosis of odontogenic mesenchymal cells and to analyze its possible...