Analysis Of Differential Expressed Gene Profiles In HGECs And HPDLCs Challenged By EMPs

The regeneration of periodontal attachment apparatus destroyed or lost due to periodontitis is a major objective in periodontal therapy. These destroyed tissues should be repaired and regenerated into physiologic status, which is termed as "true regeneration of periodontal tissues". Recently, a derivative of enamel matrix proteins (EMPs) extracted from porcine tooth buds has been introduced as a device to achieve this goal predictably. They can induce the "true" regeneration of acellular cementum, alveolar bone, and periodontal ligament where the collagen fibers arranged physiologically, which are quite different with the histological results of other methods. However, the underlying biological mechanisms are not so clear.In this study, we try to explore the genetic mechanism of EMPs in promoting periodontal regeneration by genechip methods. Two kinds of primary cultured cells, human periodontal ligament cells (hPDLCs) and gingival epithelial cells (hGECs), were used because their important role in periodontal tissues regeneration.Our study would not only map the gene differential expression profiles of hPDLCs or hGECs challenged by EMPs, but also find some key genes and/or signaling pathways, and even some new promoting factors in periodontal regeneration.We divided our study into 2 parts for the convenience of expression. Both of them shared the common investigation stratege including as follows: chosing a proper content of EMPs and test timepoint for gene chip, exploring the gene differential expression profile of hPDLCs or hGECs challenged by EMPs, and...