Peripheral Nerve Injury Induces Modification Of Genes In Rat Dorsal Spinal Cord.

Background Neurons in the dorsal spinal cord play important roles in nociception and pain. These neurons receive input from peripheral sensory neurons and then transmit the signals to the brain, as well as receive and integrate descending control signals from the brain. Many molecules important for pain transmission have been demonstrated to be localized to the dorsal horn of the spinal cord. Peripheral tissue injury-induced central sensitization may result from the altered biochemical properties of spinal dorsal horn. Although intensive research has shown that peripheral nerve injury produces robust changes in the expression of numerous genes in primary sensory neurons, peripheral nerve injury-induced modification of genes in the dorsal horn remains largely unknown. Therefore, it is important to gain a global view of changed genes in DH after peripheral nerve injury.Objective Phenotypic modification of spinal dorsal horn represents an important mechanism underlying neuropathic pain. However the nerve injury-induced molecular changes are not fully identified. To determine the molecular and gene alterations in a broader way, we are to carry out cDNA array on the gene chip mainly contained 5705 rat genes of Oligo cDNA.Methods A total 60 Adult male Sprague-Dawley rats weighing 160-200g were used for the studies of behavioral and cDNA arrays of 3,7and 14days,and 30...