Differentiation And Distribution Of Connexin And Arrhythmogenic Effects After Autologous Canine Bone Marrow Mesenchymal Stem Cells Transplantation

Differentiation and Distribution of Connexin and Arrhythmogenic effects after Autologous Canine Bone Marrow Mesenchymal Stem Cells transplantationBackground— Acute myocardial infarction as one of the most hazardous disease, is causing more and more attention. Nowadays, new therapies for AMI are growing fast from thrombolysis in early days to emergent percutaneous coronary intervention and coronary artery bypass graft. And it does saves more and more lives. But all these treatment is on the basis of early revascularization. While there is never a good solution for the necrosis cardiomyocytes, which is most important for ventricular remodeling process. Until recently, new therapy based on stem cells transplantation had thrown a light on the prognosis of many end stage diseases. There are many reports about the differentiation of stem cells and its derivatives into myocardial cells, smooth muscle cells and endothelial cells, expression of cardiac specific contractile proteins and the encouraging therapeutic effect. But there are also some reports about its severe arrhythmogenic effects. So the study try to observe whether the transplanted mesenchymal stems cells has some effects on the arrhythmia genesis after local injection to the cardiac muscle, and the differentiation and spatial distribution of Connexins, which is important for arrhythmia genesis after myocardial infarction.Part IThe purification and culture of canine bone marrowmesenchymal stem cells in vitroObjectives—to investigate the appropriate and convenient methods...