DNA Microarray-based Transcriptomics Study Of Yersinia Pestis In Response To Various Conditions

Background: Plague is an ancient deadly disease caused by Yersinia pestis, which is one of the most devastating diseases in human history. Streptomycin, chloramphenicol and tetracycline are traditionally considered as the most effective antibiotics for the treatment of plague. Cases of plague can be normally controlled by timely antibiotic administration. The development of antibiotics has significantly lowered the mortality. Plague remains a significant public health concern due to its stable enzootic foci, respiratory transmitability and the emergence of multidrug strains. Therefore, it is very important to provide insight into mechanism of antibiotic, physiology of Y.pestis.The growth of Y. pestis within macrophage after early-stage entry into host is considered as a key step for further infection and pathogenesis. Other members in our lab have established experimental system to determine the global gene expression profiling of Y. pestis under different environment stresses in vitro, such as temperature shift, heat shock, cold shock, hyperosomotic, high-salinity, low Mg²⁺, low Fe²⁺, antibacterial peptide, low pH and H₂O₂ stimulant, they have demonstrated some specific genes of Y. pestis from genome wide transcriptional responses to those stresses. Y. pestis also confronted with a nutrient-deficient environment in their life cycle, especially during living in macrophages. In this study, we investigate profiling of the bacterium in different nutrient environment in vitro. Then we investigated the transcriptional profiling of Y. pestis and macrophage after the infection with 30 min in vivo. All results above will give us a database for understanding molecule mechanism of bacterial survival in macrophage.Methods: A whole genome DNA microarray that contains 4005 annotated genes of Y. pestis was used to investigate the gene expression profile of the bacterium in response to different stresses in this study. First, we investigated the transcriptional profiling of Y. pestis in response to three different antibiotics treatment following exposure to 10 × MIC concentration of the...