The Characteristics Of Molecular Cytogenetics In Patients With Chronic Lymphocytic Leukemia

ObjectiveTo explore the characteristics of molecular cytogenetics in patients with chronic lymphocytic leukemia (CLL), evaluate the expression of proliferative antigen Ki-67 and apoptosis-antagonizing protein Bcl-2 as well as their clinical significance in CLL, and analyze the influence of factors on the prognosis of CLL.MethodsInterphase fluorescence in situ hybridization (FISH) was used to detect trisomy 12, deletion in 13ql4 and 17pl3, and IgH rearrangement in 104 previously untreated patients with B-CLL; Immunohistochemistry was used to measure the expression of Ki-67 antigen and Bcl-2 protein for 29 cases of CLL; Clinical and laboratory data of 40 patients with CLL were analyzed and Kaplan-Meier was used for survival time. The data of clinical and laboratory included ages, lymphocyte count, Binet stages, lactate dehydrogenase (LDH), p2-microglobulin (β2-MG), Ki-67, Bcl-2, ZAP-70 (zeta-associated protein - 70), CD38 and molecular cytogenetic aberrations of+12, del(13q14), del(17p13) and IgH rearrangements.Results(1) Out of the 104 patients, 76 (73.1%) had at least one kind of molecular cytogenetic aberration and 10 (9.6%) with 2 abnormalities. Trisomy 12 was found in 18 (17.3%) cases, 13ql4 deletion in 45 (43.3%), 17pl3 deletion in 13 (12.5%), and IgH rearrangements in 10 (9.6%) cases. The percentage of cells with +12 ranged from 4.0% to 34.0%, and 12.0% to 93.0%, 6.0% to 80.5%, and 10.5% to 66.5% for...