The Study Of Downregulation Of Survivin Gene In Breast Cancer Cells By Shrna To Enhance Apoptosis And Reverse Drug Resistance

Objective To investigate the expressions of survivin gene in breast cancer cell line MCF-7 and its drug-resistance cell line MCF-7/Adr and to explore the distribution of cell fractions of the two cell lines in cell cycle.Materials and Methods Human breast cancer cell line MCF-7, the drug-resistance cell line MCF-7/Adr and leukemic cell line K562/AO2 were cultured in RPMI 1640 medium containing 10% heat-inactivated calf serum. A survivin primer was designed and synthesized. Total RNA was extracted and semi-quantitative RT-PCR was performed to detect survivin mRNA expressions in MCF-7 and MCF-7/Adr cell with K562/AO2 as positive control and β-actin as internal control. Immunocytochemistry stainining (SABC) was used to determine the expression of survivin protein with K562/AO2 as positive control. Distribution of the cell fractions in different phases of cell-cycle and proliferation index were analyzed by Flow Cytometry.