The Dynamic Changes Of ICAM-1,IL-18,IL-10 Levels In Thyroid, Spleen, And Mesenteric Lymphnode During Early Postburn Stage In Severely Burned Rats

Introduction:Hosts after severe burn injury are known to have a defect in the Th1 immune response and are susceptible to bacterial infections. Burn injuries as well as skin damages are often associated with immune suppression and immune dysfunction and often cause multiple organ failures.Cytokines are important modulators of physiologic alterations after thermal injury. Patients with severe trauma or polytrauma frequently acquire alterations in immune functions which are correlated to dysbalanced cytokine synthesis.Indeed, an increase in the level of circulating cytokines has been documented after thermal injury. However, the mechanism of the increase has not been clarified. Studies have shown that susceptibility to sepsis after severe injury correlated with disordered production of T-helper 1 (Thl) cytokines (interleukin-18 (IL-18) and interferon-gamma(IFN-gamma) and a persistence of T-helper 2 (Th2) cytokines (IL-4 and IL-10). The mechanisms responsible for this effect are not clear.Objective:We determined cytokine levels in immune organ's tissue as well as the serum levels after thermal injury to identify the possible mechanism of immune dysfunction postburn.1. To study the pathological changes in immune organs such as spleen,thyrum and mesenteric lymphnode in early postburn stage.2. Flow cytometric analysis (FCM) was used to investigate the cellular apoptosis of the pleen, lymphnode and the thyrum with large burns.3. To observe the postburn changes in the serum levels of sICAM-1, IL-10 and IL-18 during early postburn stage.4. To determine the expression levels of ICAM-1, IL-10 and IL-18 in spleen,thyrum and mesenteric lymphnode.