Application Of Fish In Reproductive Gentics

PART 1: ANALYSIS OF SEX CHROMOSOMES OF TRIPRONUCLEUS ZYGOTES DERIVED FROM IN-VITRO FERTILIZATION AND INTRACYTOPLASMIC SPERM INJECTION CYCLES IN HUMANObjective: To analyze the implantation value of tripronucleus zygotes derived from in-vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) by examining the sex chromosomal aneuploid with fluorescent in situ hybridization (FISH) technique.Methods: Tripronucleus zygotes (3PN) from IVF and ICSI were examined by FISH with X/Y dual color centromere probes. The signals were counted and analyzed by statistic software.Results: A total ratio of hybridization was 73.53%; in 3PN after IVF, the ratios of triploid, diploid and monoploid were 92.31%, 3.85% and 3.85%, respectively. Three types of sex chromosome karyotype were found: XXX, XXY and XYY; in 3PN after ICSI, the ratios of...