Experimental Research On Tissue Engineering Bone With Xenogeneic Cancellous Bone Scaffold

Scaffold material are key point in bone tissue engineering , which can not only affect the biological conducts of cell and the efficiency of cultivation , but also decide the adaptation, integration ,and reparation in the tissue after transplantation . Bio - derived scaffold material with the natural structure of three-dimensional micropore is similar to bone of human, meanwhile it is conductive to the attachment and proliferation of osteoblasts as well as reconstruction of bone tissue . Moreover it has been a significant interest of research of scaffold materials. Currently pig - person heterotransplantation has been widely regarded as one of possible ways to satisfy the serious need of supplying organs. Researches on scaffolds of acellularized porcine aorta petal and porcine corium have been reported in tissue engineering. Hereby porcine cancellous bone has been handled with physics and chemistry in my experiments in order to evaluate the structural properties, cellular biocompatibility, transplantation immunity in vivo, and bone regeneration, which can consequently offer experimental evidences for clinical application.Chapter Ⅰ : The preparation on the porcine cancellous bone and the Observation of structural feature of the porcine cancellous bonescaffold material.Objective: To detect the structural feature of the porcine cancellous bone scaffold material. Material and Methods: The porcine cancellous bone was gained through physical and chemical disposals. The scan electron microscope (SEM), image analysis, liquid-noncurrent force weight was used to exeamine the morphological feature, aperture, porosity respectively. Results: The appearance of the porcine cancellous bone scaffold was white cancellous feature, the porosities were connected each other under SEM, the average aperture was 387.54±21.60μm, the...