Differential Expression And Function Of Slit And Robo As The Axonal Guidance Cue And Receptor Following Injured Peripheral Nerve

A breed of extracellular glycoprotein secreted by glia in nervous system, Slit is capable of repelling axon in growth, inducing cell migration, and facilitating the growth and branching of axons. As a member of Ig superfamily, the cell adhesion molecule Robo interacts with Slit's exclusive function towards growthcone, participates in guiding the growth direction of developing central nervous, and controls the fasciculi. So far, little is known about Slit's and Robo's distributions and their functions in peripheral nervous system of adult animals. Still, unclear is the cell and molecule mechanism with regard to the regeneration characteristics of peripheral nerve.Objective: To investigate into the expressions and changs of Slit and Robo in intact and injured adult rat's spinal cord, DRG and nerve trunk with sciatic nerve transection or dorsal root rhizotomy animal models, to explore the roles that they play in the regenerating process of injured nerve, and to disclose the physiological functions of Slit and Robo as well as their molecular mechanism regarding the regeneration characteristics of peripheral nerve.Methods: After the right side sciatic nerve transection or the right side dorsal root rhizotomy and surviving respectively 3, 7, 14, 21, 28 days in Sprague-Dawley rats, the expressions of Slit1, Slit2, Robol,...