Study On Capillary Electrophoresis Interaction Analysis And Its Applications

Molecular interactions exist widely in chemical, biochemical and environmental systems. Bioactivities and biological functions are based on these molecular interactions. Capillary electrophoresis has unique advantages of efficient separation, fast speed and small amount of samples required, in studying molecular interactions. Therefore, studies and applications of molecular interactions by capillary electrophoresis are of significant importance for chiral pharmacology, environmental toxicology, drug discovery and biomedical engineering.The interactions can be characterized by many parameters such as binding constants, binding sites and free energy etc. Hoewer, the binding constants is one of the most important parameters to characterize the interactions.In this thesis, an approach called capillary electrophoresis interaction analysis (CEIA), especially for interactions among those neutral-electronic molecules in complex systems, was established. Binding constants of small molecules with biomacromolecules, with bacteria and red blood cell are determined by CEIA. Based on the principle of molecular interactions, chiral separation of citalopram hydrobromide was successfully achieved,by using amylose,dextrin and maltose as chiral additives;The mechanism of chiral recognition by maltose was studied further. The main contents of this thesis are listed below:(1) According to the different mobilities caused by sodium dodecylsulfonate, interactions of neutral host molecule 18-crown-6 with p-nitroaniline and 2-aminopyridine were studied by CEIA respectively. The CEIA method of neutral molecules was established. The binding constants of host molecules with guest molecules were determined by capillary electrophoresis peak-shifting method.(2) The CEIA method was expanded to determine molecular interactions of components in complex systems,which was able to evaluate binding abilities of an interested component with all other components within one test. The binding constants of bioactive components from Rhubarb such as Emodin, Aloe-emodin and Chrysophanol withβ–cyclodextrin and hydroxypropyl-β-cyclodextrin were determined by this method.(3) The partial-filling-self-marking technique (PFSM) was employed for the interaction analysis of ephedrine, caffeine and acetaminophen with Bovine Serum...