Dextran-Coated Pfob Emulsion

Objective:Our overall goal is to develop a targeted carrier molecule that can carry multiple copies of the same or different ligands/reporters. The purpose of the present study was to demonstrate the receptor-binding properties of DTPA-Mannosyl-Dextran in vitro. We tested the hypothesis that FITC-DTPA-Mannosyl-Dextran mimics the receptor binding properties of native ligands to the receptor, mannose binding protein. This receptor is a C-type lectin that requires calcium for ligand binding.Method:DTPA-Mannosyl-dextran (MW=35,800, 56mol mannose units per dextran) was labeled with FITC (spell out). Macrophages J-774 (American Tissue Culture Company) cultured on coverslips in a 24 well plate were fixed with 3.0% paraformaldehyde (w/v in PBS buffer) for 4 minutes to block antigen internalization. This treatment has been previously shown to preserve cell surface-associated enzyme activity without exposing lysosomal enzymes. The nucleus of macrophages was...