Inhibitory Effect Of Broad-Spectrum Deoxyribozyme On Respiratory Syncytial Virus In Mice With Different Immune Functions

Background and ObjectiveRespiratory syncytial virus (RSV) is the worldwidly leading cause of acute lower respiratory tract infections in young children. All the population are susceptibility to RSV infection, and premature, transplant recipients, congenital heart disease patients, immunocompromised individuals are vulnerable for severe illness and even death. RSV is a highly variable RNA virus including A and B subgroups with multiple viral strains. Primary infection of RSV does not elicit efficient immunization, so reinfection is common. The mutation of RSV hinders the prophylaxis and treatment, and neither a reliable vaccine nor a specific anti-RSV agent has been currently available.10-23 deoxyribozyme (DZ) is the DNA molecule selected from a combinatorial library of sequences in vitro and capable of specifically cleaving RNA molecules. It is a novel approach of genetic therapy and widely applied in suppressing a variety of target genes in infections, tumour and genetic diseases. In previous studies, we have already designed a serial of deoxyribozymes targeting messenger or genomic RNA of RSV, and demonstrated the anti-RSV activities of DZ604,DZ8269 and DZ1133. DZ1133 targeting vital and conserved sequences of RSV nucleoprotein genomic RNA particularly inhibited the replications of A and B subgroups...