Study On Quantification Of Cerebral Perfusion Under Different Pathophysiological Conditions With Real-time Contrast Enhanced Ultrasound

[Background]In current clinical practice, noninvasive assessment of cerebral blood flow (CBF) can be achieved by use of various techniques, such as CT, MRI, and single photon emission CT (SPECT). These techniques, however, are ill suited for measurement of CBF at the bedside or in the operating room. Furthermore, they cannot be used repeatedly, in rapid succession, to measure changes in CBF that may occur in the natural course of events or in response to treatment. Contrast-enhanced ultrasound (CEU) is a new developed technique to evaluate various substantive tissues, such as myocardium, skeletal muscle, and kidney. This technique, using small gas-filled microbubbles, can provide an assessment of microvascular blood volume (MBV) as well as red blood cell velocity (RBCv). But, few reports about this topic in dom were seen.[Objectives]The aims of our study were to evaluate the feasibility of CEU used in assessing and monitoring of CBF, and to detect the changes of cerebral perfusion of the dogs under different pathologic conditions such as normal, hypercapnia, hypocapnia, acute cerebral ischemia, drug-induced acute hypertension and hypotension.[Methods]1. Methodological study on quantification of CBF with CEUCerebral perfusion was assessed in six dogs through a craniotomy (4cm×2.5cm in left parietal bone) with CEU at normal and during hypercapnia and hypocapnia. The blood flow of common carotid artery was continuously monitored with Doppler flowmeter. CEU was performed with Sequoia 512(ACUSON) capable of performing contrast pulse sequencing technique (CPS). The ultrasound transducer was fixed in a...