Studies On The Structures And Biological Activities Of Chemical Constituents From The Soft Coral Sinularia Dissecta And Marine Sponge Aplysina Aerophoba In South China Sea

In our ongoing program to search for natural products with unique structural features and pronounced biological activities, the soft coral Sinularia dissecta and marine sponge Aplysina(Verongia) aerophoba were collected from Sanya city, Hainan island, South china sea. Repeatedly column chromatography was performed for the isolation and purification of their MeOH extracts. 52 pure compounds were obtained from these two marine organisms. Their structures were determined by extensive spectroscopic analysis in association with physical and chemical properties, as well as comparison of their spectral data with these reported in literatures. Most of these compounds were screened for their antitumor(HL-60 human leukemic cell, A-549 human lung cancer cell, PC-3MIE8 human prostate cancer cell, BGC-823 human gastric cancer cell, MDA-MB-435 human breast cancer cell, Bel-7402 human hepatoma cancer cell, Hela human cerrical cancer cell), COX-2 inhibition, ACE inhibition and PTPIB inhibition activities.37 Compounds, namely 3β- Acetoxy -1α, 11 α - dihydroxygorgost - 5 - en- 18-oic acid (1), Gorgost-5-en-1α, 33, 11α, 18 - tetrol (2), 18 - Acetoxy -1α, 3β, 11α - trihydroxygorgost - 5 - ene (3) ,24 (S) - 3 3 - Acetoxy -1α, 11α - dihydroxyergost - 5 - en - 18 - oic acid (4),24 (S) - Ergost - 5 - en -1α, 3β, 11α, 18 - tetrol (5), 24 (R) - 3 - Acetoxy - 1α, 3β, 11α -trihydroxyergost - 5, 22 (E) - dien - 13 - oic acid (6),Dissectolide (7), Dissectanol (8), 24 - Methyl enecholest -5-en-1α, 3β, 11α, 18- tetrol- tetracetate (9), 24 - Methylenecholest -5-en-1α, 33, 11α, 18- tetrol (10), Gorgost -5-en-1α, 3β, 11α - triol - 13 - carbaldehyde (11), Gorgost -5- en-1α, 3β, 11α - triol - 18 - oic acid (12), 24 (S) - Ergost - 5 -en-1α, 3β, 11α - triol - 18 - oic acid (13), 24 - Methylenecholest - 5 -en- 1α, 3β, 11α - triol (14), 24 - Methylenecholest - 5 - en - 1α , 3β, 11 α - triol - 18 - oic acid (15), 24 - Methylenecholest - 5 - en - 1α , 3β, 11 α , 18 - tetrol - 18 - acetate (16), 24 - Methylene - 3β- acetoxy -1α, 11 α...