| Looking for drugs from the sea has become a new trend in pharmaceutical research for half of the century in countries along the coast. Scholars of many countries have isolated and identified antineoplastic agents from marine lives using modern scientific methods. This founds the foundation of development and exploitation of new antineoplastic drugs. For the past several years, many antineoplastic materials with original structures have been identified from marine organisms. And this presents a marvelous prospect in drug discovery area. Screening and extracting antineoplastic agents with unique chemical constitution from marine lives and their metabolites becomes more and more important. Arca granosa Linnaeus, commonly called bloody clam, is widely distributed in the inshore provinces of China. It is quoted in the Medical Compendium named"Yi Lin Zuan Yao"that Arca granosa Linnaeus'helps to repair heart function, dispel gore, eliminate haematomas and reduce restlessness'. The flesh of Arca granosa Linnaeus has health-care functions, whose active components along with its physiological function have aroused popular attention. In this study, polypeptides from Arca granosa Linnaeus are isolated. And its antineoplastic effects and mechanisms in vitro and in vivo are fully investigated.The polypeptides from Arca granosa Linnaeus are isolated by gel filtration chromatography. Its protein content is 67.32±3.45 mg/ml with molecular weight mainly from 1000 to 5 000 Da. The antineoplastic effects and immune enhancements of the polypeptides are observed in vitro and in vivo. Fourther more the antineoplastic mechanisms are investigated in human tumor cell lines (A549) and female C57BL/6 mice bearing Lewis carcinoma by cellular morphology, single cell gel electrophoresis, flow cytometry, immunochemistry, RT-PCR and western blot respectively. Finally, the toxicology of the polypeptides is also evaluated.Our results indicate that the polypeptides can decease the DNA synthesis and total... |
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