Study On The Immunologic Properties Of HPV16 E7 Protein And Preparation Of Monoclone Antibody Against It

ObjectivesHuman papillomavirus type 16(HPV16) is the most important cause for cervical cancer. Over 90% of cervical cancer patients have ever infected with HPV, more than 60% of which were texted as HPV16. After the genome of HPV16 integrated into celluar chromosome, HPV16 E7 protein can be persistently expressed with high level in it. HPV16 E7 protein can bind effectively with Rb, which is one of the most important tumor suppressor proteins for cell. In normal condition, Rb is bound with E2F, which is a major transcription initiation factor for eukaryocytes. Once at the time Rb is bound with HPV E7, not only will Rb protein be degradated quickly, but also E2F will be released greatly, it will finally enhance expression of many proteins which are not expressed at normal. All of these can bring the cell checkpoint from G1 to S phase, which resulting in the cell being a condition named malignant transformation. So it is a popular view looking HPV16 E7 as a tumor specific antigen(TSA) for HPV16 related cervical cancer and it is also confirmed as an ideal target for the immunotherapy on HPV16 related cancer.Up to date, PCR is a main weapon used to find HPV infection, including HPV16, which is most important in clinical. But as we know, PCR technique is greatly dependented on the equipment and it must be performed under a relatively seperated and clean environment. Otherwise it will give us many false positive information. On...