Effects Of Hypoxia On Osteogenic Differentiation Of Bone Marrow-derived Mesenchymal Stem Cells In Vitro

Bone remodeling is the biologic fundament of orthodontic movement , and osteoblasts play a key role in the bone remodeling . Nevertheless , it is incompletely understood that how bone responds to mechanical force during the orthodontic movement . When decreased blood supply and hypoxia were observed on the pressed side not tensional side , some investigators speculated the changes of oxygen tension could be the potential trigger of bone remodeling . It was verified that mesenchymal Stem Cells(MSCs) were precursor cells of osteoblasts , MSCs are capable of differentiating into osteoblasts induced by osteogenic supplements in vitro . The effects of hypoxia on osteogenic differentiation of MSCs is unknown exactly , neither are the involving mechanisms.Objects: the focus of this current study is to explore such effects on Osteogenic Differentiation of MSCs by hypoxia and potential regulatory mechanisms , to provide the experimental evidences for the illumination of orthodontic movement mechanisms .Methods: We established a model of osteogenic differentiation with rBMSCs . rMSCs were isolated and purified in vitro , and the culture dishes with seeded MSCs were placed inside the multigas CO₂ incubator (Jouan from France) . Experiments employed several advanced methods , such as Fluorescent labeling method , Alkali phosphatase activity assay , RT-PCR and Western blotting.Combing these assay results , we can understand...