Effects Of High Glucose, Insulin On Expression Of Glucose Transporter 4, Cbl-associated Protein And Changes Of Cytoskeleton In Rat Glomerular Mesangial Cells In Vitro

OBJECTIVE: To investigated the effects of high glucose, insulin on expression of Glucose Transporter 4 (GLUT4), Cbl-associated protein (CAP) and cytoskeleton protein F-actin of glomerular mesangial cells (GMCs) in order to explore the function of Glucose Transporter 4, Cb1-associated protein and F-actin in the genesis and development of diabetic nephropathy (DN). METHODS: Cultured 1097 rat glomerular mesangial cells were divided into 8 groups: control, 10^-9M insulin, 10^-8M insulin, 10^-6M insulin, high glucose, mannitol, high glucose plus 10^-6M insulin, high glucose plus 10^-9M insulin. Expression of GLUT4 and CAP mRNA was measured by RT-PCR and immunohistochemistry method, F-actin was stained by rhodamine-pholloidin and the fluorescent intensity was calculated by image analysis system.RESULTS: The expression of GLUT4 mRNA and protein, CAP mRNA exists in normal glomerular mesangial cells. The expression of GLUT4 mRNA and protein, CAP mRNA level was decreased in high glucose group;...