The Study Of Different Immunosuppresants On Expression Of Fractalkine, CX3CR1 In CAN

Objective Some recent studies have suggested that Fractalkine/CX3CR1 system may contribute to the pathogenesis of vascular and tissue injury in chronic allograft nephropathy (CAN). It is still unknown of the influence of different immunosuppresants on expression of Fractalkine/CX3CR1 in CAN. This study is to investigate what role Fractalkine/CX3CR1 play in CAN and whether there are different effects among different immunosuppresants on expression of Fractalkine/CX3CR1 in CAN.Methods The SD→Wistar rat accelerated kidney sclerosis model was performed following the procedure of Kamada with our modification. Before the transplantation, the kidney was preserved one hour in 0-4℃ heparin sodium chloride solution for reinforcement of the injury of cold ischemia. The recipient rats were divided into 6 groups (each group n=8). Group A: pseudo-operation group; Group B (control group ): CsA 10mg·kg^-1·d^-1×10d, followed by vehicle; Group C: CsA emg·kg^-1·d^-1; Group D:FK506 0.15 mg·kg^-1·d^-1 Group E: MMF 20 mg·kg^-1·d^-1 Group F: RAPA 0.8mh·kg^-1·d^-1...