Antigenicity Analysis Of Rabies Virus GP And NP And Genetic Variance Research Of Rabies Virus GP Gene

Rabies virus, the etiologic agent of fetal neurological disease, is composed of five structural proteins, both of the glycoprotein (GP) and the nucleoprotein (NP) are believed the decisive fectors for rabies diagnosis and classification and for rabies neurotropic, pathogenicity and antigenecity. Many studies have shown antigenic differences of NP and GP in some strains of rabies viruses which came from different vectors or from different areas. According to these differences it is possible to divide them into group and identify the variants from them, in this way we can find out the clue of epidemic sources. Further study and analysis of the GP gene is very important for understanding of the mechanisms of neurotropic, pathogenicity and the antigenicity of rabies viruses.Twenty one MAb-RNPs by immunofluorescent method and ten MAb-Gs by neutralization test were used to analyze the antigenic structure of four Chinese rabies viruses: human rabies vaccine strain(aG), one attenuated fixed strain(CTN-181) and two street viruses isolated from human in Ning Xia province. On this basis, the G gene of the four viruses are sequenced: first, double-stranded PCR products of the template DNA were made with specific primers by RT-PCR, then direct dideoxynucleotide sequencing of the PCR products was performed and each fragment was sequenced in both directions, finally linked every segment together and got the complete GP...