Expression Of Hantaan Virus Envelope Glycoproteins G1 And G2 In E.coli And Immunogenicity Of Expressed G1, G2 And Nucleocapsid Protein (NP)

Hantaan virus , the etiologic agent of hemorrhagic fever with renal syndrome, is the prototype of the Hantavirus genus of the Bun-yaviridae family. It has a three-segment, single-stranded RNA genome, whose segments are designated as large (L), mediume (M) and small (S), which encode the structural proteins, RNA polymerase, envelope glycoproteins (G1, G2), and nucleocapsid protein (NP) , respectively. It has been reported that the envelope glycoproteins can elicited neutralizing antibodies and protective cell-mediated immune response in animals, the nucleocapsid protein only elicited protective cell-mediated immune response.Three expression vector plasmids (PBV220, PKK223-3 and PGEX-4T-1) were used to express the envelope glycoproteins Gl and G2 of hantaan virus in E. coli. First,the coding regions of Gl and G2 were amplified by using PCR, and cloned into the PCR product cloning T-vector, and then it was cutted off using restriction endonu-...