Study Of Expression Of VEGF Transfected In Salivary Glands And Its Effect On Intra-oral Wound Healing

Maxillofacial injury frequently occurred in normal and wartime.Vascular endothelial growth factor(VEGF),which takes part in wound healing process,is a kind of lately disco(?)d cell growth factorof angiogenesis.On the base of animal models of maxillofacial blast injur(?) (?)ound,we dynamic observed the changes of bFGF,VEGF and its (?) post-wound tissue byimmunohistochemistry and image analysis technology (?)karyotic expression vector—pBKCMV-VEGF₁₂₁ was constructed by molecule clone and gene transfection technology . With the characteristic of intra-oral environment, the extra-VEGF cDNA was transfected in maxillary glands guiding by cation liposome and expressed normally in it. so,the content of VEGF in spittle raised and me process of intra-oral was shortened. The expression of VEGF and its mRNA in maxillary glands,spittle and injured tissue after gene transfection were detected by immunohistochemistry,ELISA,RT-PCR, etc. Also, the affection on intra-oral wound healing was observed Result:The expression of VEGF and its receptor in maxillofacial blast injury and incised wound were similar, it is higher than pre-injury group in evidence at post-injury 2-7d. Sameness, the content of VEGF in wound fluid was higher than pre-injury group at post-injury 1-7d,it reached the peak value at post-injury 7d;but the contentof bFGF arrived at the...