Expression Of Multidrug Resistance-related Molecules: GST-π, MRP, LRP, MDR1 And MGr1-Ag In Gastric Cancer

AIM: Carcinoma usually shows little or no response to chemotherapy and multidrug resistance (MDR) is widely thought to be a major cause of the failure in cancer chemotherapy. The detection of MDR marker is therefore worthwhile to study the mechanisms of MDR and predict response to chemotherapy in patient with carcinoma. Gastric cancer is one of the most common malignances in our country. The previous studies have examined MDR marker in gastric cancer, but the majority of investigation in patients have focused on a single parameter, mainly multidrug resistance gene1 (MDRl)/P-glycoprotein(P-gp) or multidrug resistance-associated protein(MRP). The purposes of this study is to detect the expression of glutathione S-transferase pi(GST-Ï€), MRP, lung-resistance protein(LRP),MDR1 and MGr1 antigen(MGr1-Ag) in the patients with primary gastric cancer, who had not received prior chemotherapy, and evaluate the correlation between GST-Ï€, MRP, LRP, MDR1 and MGr1 -Ag.Material and methods: The expression of GST-Ï€mRNA, MRP mRNA, LRP mRNA and MDR1 mRNA in carcinoma tissue and the adjacent non-cancerous tissue from 50 patients, who have suffered from primary gastric adenocarcinomas and had not received prior chemotherapy, was examined by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). The expression of...