A Study On Detection Of Enteroviruses In Water By RT-PCR For Evaluating Inactivation Efficacy And Virucidal Mechanisms Of Different Disinfectant Methods

The present studies were undertaken to investigate the feasibility of application of reverse transcription polymerase chain reaction (RT-PCR) to evaluate the inactivation efficacy of enteroviruses in water, and to discuss the virucidal mechanisms of heat, ultra-violet, chlorine and iodine by way of comparison detective results for antigenicity and nucleic acid with effectively of Poliovirus type 1 (PV1).Part one - Establishment of cell infection model for inactivation of Poliovirus in water.1. Screening and selecting suitable virus strains and cell lines. We compared the results of cytopathic effects (CPE) , 50% tissue culture infective doses (TCID₅₀) , plaque forming units (PFU) and dot immunobinding assays (DIBA) after 5 virus strains were separately propagated for 3-5 days in 5 cell lines. Determination of CPE and TCID₅₀ showed that (1) the virulence of 5 strains to the same cell line ranged from the highest to the lowest as Henan, Hebei >Brunhide, Mahony >Sabin; (2) The sensitivities of 5 infected cell lines to the same strain were the following range : Hep-2>BGM>Hela, Vero>RD. Detection of PFU and DIBA indicated Henan strain was inspected more easily than Brunhide strain after both strains were propagated in Hep-2 cells. So PV 1 Henan strain and Hep-2 cell line were appropriate to the experiment of inactivation of PV in water.2. PV 1 propagation and purification with polyethylene-glycol (PEG) 6000 precipitation and...