Construction Of Eukaryotic Expression Recombinant Plasmids Containing Gene Fragments Of Several Plasmodium Falciparum Blood-stage Antigens And Their Immunity

Malaria is still one of the most important infectious diseases in the world. Each year there is 2 million deaths due to malaria; most of death is caused by Plasmodium falciparum. The appearance and rapid spread of drug-resistant parasites and insecticide-resistant mosquitoes have serious impaired the effectiveness of these common tools for malaria control. The need for an effective vaccine is thus urgent. A new kind of vaccine, nucleic acid vaccine(mainly including DNA vaccine), applies for a strong tool for this goal. Since 1994, Dr.Hoffman SL et al. have been doing research work on pre-erythrocytic stage DNA vaccine, and now they get very excited results, but the most effective malaria vaccine must be multistage (including pre-erythrocytic stage, blood-stage and sexual stage), multivalent vaccine. Since 1995, supported by TDR and Chinese Military Medical and Hygiene Foundation, our department has been working on Plasmodium falciparum blood-stage DNA vaccines.In this report, several gene fragments from Plasmodium falciparum blood-stage antigens, which have been confirmed to induce protective immunity, were chosen to construct DNA vaccines. These fragments are gene coding for 19kd (block 17) of Merozoite surface protein 1 (MSP1), gene coding for 42kd protein (block 15-17) of MSP1, gene coding for Histidine rich protein 2 (HRPII) and a synthetic hybrid gene (HG) encoding...