Empirical Study Of Local Profound Hypothermia Freezing In Peripheral Nerve Injury

Background: Immunological reaction will happen when the nerve is injured, which will effect regeneration and functional recovery of nerve. For knowing the relation of Immunological reaction and peripheral nerve injury, and the experimental nerve regeneration result of different immune suppression methods, we take contrast tests. Because nervous peripheralis; peripheral nerve have blood-nerve barricade, it can keep lymphocyte and antibody out of essence of nervous tissue, it has not the express of major histocompatibility complex antigen and the presence of antigen presenting cell, it is regarded as immunme-privileged sites for many years. But during the research of home and abroad for nearly years, many immune reaction will take place after the injury of nerve. Method: sixty adult SD mice, cut sciatic nerve of extremity of one side. Some twenty mice's nerve broken ends are freezed with profound hypothermia, and are anastomosed nerve after rewarming (A group). Some twenty mice with the hypothesis of exactly anastomosed nerve, are put FK506 slow-released membrane around the nerve anastomotic stoma which will slow-releasing. It can accommodate (B group). As contrast of the others, they are only exactly anastomosed nerve. After 12 weeks later, I take them togeneral state, index number of extending claw reflex, describtion of stoma, Electrophysiology determination, The sciatic nerve was observed under microscope and transmission electron microscopy, and myeline sheath thickness, axon diameter and axon count per HP were measured with image pattern analysis. nerve fiber density ratio enumerate per surface, Electronmicroscope: the thickness of myelin sheath, the construction of layers,...