Study On The Bioactive Constituents Of Centella Asiatica And Cucubalus Baccifer

Centella asiatica has been used for treatment of skin diseases, diarrhea, eye diseases, inflammation and leprosy in China, India, Sri Lanka, Africa and oceanic countries. Many constituents has been reported, such as triterpenoid saponins, polyacetylenes, flavone, sterols and lipids. In order to search the active compounds, we began to study on the chemical constituents from C. asiatica. By column chromatography, including Silica gel, Sephadex LH-20, Toyopearl HW-40 and preparative HPLC, 28 compounds were isolated from the EtOH extract of C. asiatica, among them, two new (isoasiatic acid 1, isoasiaticoside 2) and 22 known compounds (3-22) were identified by 1H-NMR,13C-NMR and 2D-NMR spectra.Cucubalus baccifer L. is a Chinese folk herb used for treatment of nephritis, hydropsy, bone-fractures, arthritis, pulmonary tuberculosis and scrofula. Oligosaccharides, tocopherol, phytoecdysterones, norsesquiterpenoid have been reported from C. baccifer. By column chromatography, including Silica gel, Toyopearl HW-40 and preparative HPLC, 46 compounds were isolated from the EtOH extract of C. baccifer and 15 known compounds were elucidated by 1H-NMR,13C-NMR and 2D-NMR spectra. In addition, the identified 15 compounds were first isolated from this genus .The EtOAc, BuOH extracts of C. asiatica, and PE, EtOAc, BuOH extracts of C. baccifer as well as isolated compounds, were screened by antitumor experiments. The EtOAc extracts of C. asiatica showed some antitumor activity. Compounds 6, 7, 9, 10, 13, 14, 17, 18, 21 and 33 have some activity to inhibit Hela cell. Compounds 7, 9, 11, 14, 17and 33 have some activity to inhibit A549 cell.In the lymphocyte transformation experimation, compounds 1, 2, 13 and 30 showed a significant distinction to the concanavalin (Con A) control group (p<0.01, n = 6) and positively showed immunosuppressive activity.In this paper, 15 flavone molecular for antihistamine were selected to dock into carbonic anhydrase by using the Dock program. The results showed that tricin, quercetin and kaemferol were better than other compounds for antihistamine.