| In this thesis, a home made capillary electrophoresis-laser-induced fluorescence (CE-LIF) system has been established for application in single cell analysis and multidrug resistance research to elucidate the relative mechanisms of multidrug resistance. Leukemia cell lines (K562) were selected as the target cell line of investigation and carbon nanotubes were investigated as drug transporter for anti-cancer research. The main research contents are as following:Firstly, a method of combination CE-LIF with flow cytometry was established for high throughput determination and quantitation of fluorophores Rhodamine 123 in single intact K562 parental cell line (K562S) cells. The results of CE-LIF and flow cytometry were correlated. The membrane properties of K562S cell including fluophor transport rate and apparent permeability coefficient were further quantitatively characterized.Secondly, a method was established to characterize P-glycoprotein on single K562 cells by CE-LIF. A resistance factor so called multidrug resistance multiple was introduced to evaluate the multidrug resistance difference between cell lines. The results of single cell analysis, multi-cell analysis and flow cytometry were consistent.Thirdly, the possibility of oxidized multi-walled carbon nanotubes as drug transporter was evaluated. They were derivatised with fluorescein isothiocyanate to form carbon nanotube probe (CNTP). Analyses of CNTP in K562 multidrug resistance cell (K562A) and K562S cell using both CE-LIF and flow cytometryshowed that CNTP could transverse the cellular membrane without being pumped out by P-glycoprotein.Fourthly, the distribution of CNTP in yeast was quantitatively determined by CE-LIF. The time and temperature dependent influx patterns of CNTP in yeast were obtained. The apparent permeability coefficient for influx of CNTP into yeast was calculated, which suggested that CNTP might permeate into yeast through endocytosis.
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