Study On The Immunity Relationship Between Epithelial Ovarian Tumor And CD4～+CD25～+ Regulative T Cell

Ovarian tumor is one of the most popular gynecological tumor. The survival rate of 5 years is 30%. It is the one of the most popular tumor threating for women. The population of epithelial ovarian tumor in ovarian tumor is 50-70%. The research about the epithelial ovarian tumor is very important.We have known that there are regulatory T cell(Treg), but we know little about Treg. Sakaguchi found the population of CD4~+CD25~+ Treg in CD4~+ T cell was 5-15%. In 1995, they deleted the CD4~+CD25~+ Treg and found that it caused the autoimmune disease. Then they input the CD4~+CD25~+ Treg and found that it could prevent the autoimmune disease. It shown the CD4~+CD25~+ Treg can suppress the immunity. With more research about CD4~+CD25~+ Treg, it is found CD4~+CD25~+ Treg could influence the immunity balance, tolerance of translate, infection of germ, allergic response and tumor.For CD4~+CD25~+ Treg inhibit the immunity of T cell for antigens, it may be partially responsible for the lack of anti-tumor immune response, and influence the development of tumor.To investigate the changes and origin of the CD4~+CD25~+ Tregs, the influence of Treg to the epithelia ovarian tumor, we detect the CD4~+CD25~+ Treg and FOXP3 in peripheral blood mononuclear cell from the patients with epithelial ovarian tumor, the T lymphocyte function and Treg , foxp3 mRNA in thymocytes and spleenocytes of ovarian tumor model, the influence of low-dose cyclophosphamide to the T lymphocyte function and Treg, foxp3 mRNA in thymocytes and spleenocytes of ovarian tumor model. There are three parts in the article.Part I The changes of CD4~+CD25~+ Treg cells and expression of FOXP3 mRNA in peripheral blood mononuclear cell from patients with epithelial ovarian tumorWe investigated the changes of CD4~+CD25~+ Treg and Foxp3 mRNA in peripheral blood mononuclear cell from the patients with epithelial ovarian tumor, found the population of CD4~+CD25~+ Treg in peripheral blood mononuclear cell from patients with serous cystadenocarcinoma, mucinous cystadenocarcinoma was significantly higher in comparison with that of controls. The expression of FOXP3 mRNA in peripheral blood mononuclear cell enhanced in patients with tumor comparison with that of controls.Part II The changes of the T lymphocyte function, CD4~+CD25~+ Treg and expression of foxp3 mRNA in thymocytes and spleenocytes of ovarian tumor model.We investigated the changes of the T lymphocyte function, CD4~+CD25~+ Treg and expression of foxp3 mRNA in thymocytes and spleenocytes of ovarian tumor rat model, found the T lymphocyte function of spleenocytes decreased , the population of CD4~+CD25~+ Treg in spleenocytes of ovarian tumor model was significantly higher in comparison with that of controls, the expression of foxp3 mRNA enhanced. There are no obvious changes in the thymocytes.Part III The influences of low-dose cyclophosphamide to the T lymphocyte function, CD4~+CD25~+ Treg and expression of foxp3 mRNA in thymocytes and spleenocytes of ovarian tumor rat model.We investigated the influences of cyclophosphamide to the T lymphocyte function, CD4~+CD25~+ Treg and expression of foxp3 mRNA in thymocytes and spleenocytes of ovarian tumor rat model. There was no difference between low-dose cyclophosphamide administration group and ovarian tumor group of T lymphocyte function in spleen. the population of CD4~+CD25~+ Treg in spleenocytes of low-dose cyclophosphamide administration group was significantly lower in comparison with that of ovarian tumor group, the expression of foxp3 mRNA decreased. There are no obvious changes in the thymocytes. Conclusion:1. the population of CD4~+CD25~+ Treg cells in peripheral blood mononuclear cell from patients with serous cystadenocarcinoma, mucinous cystadenocarcinoma was significantly higher and the expression of FOXP3 enhanced. It may be one of the mechanism of the ovarian tumor immune escape 2. This is the first report demonstrating that peripheral induction caused the population of CD4~+CD25~+ Treg cells in spleenocytes of ovarian tumor model was significantly higher in comparison with that of controls, the expression of foxp3 mRNA enhanced.3. This is the first report low-dose cyclophosphamide reduced the immunity escape of epithelia ovarian tumor and maybe increase the curative effect of tumor vaccine.