The Study On The Changes Of TCM Syndromes Of Malignant Bone Tumor Patients And The Empirical Study On Herbs Of Invigorating Vital Energy And Promoting Blood Flow And Eliminating Phlegm Treating Tumor-bearing Mice

The total incidence rate of malignant bone tumor is low, but it oftenresults in extremity disability and even threat to life. For the past fewyears, five year survival rate consumedly raised since combined therapyincluding operation and neoadjuvant chemotherapy etc, was applied. TCM,as a part of combined therapy, manifests characteristic therapeuticeffect and potentiality on stabilizing the focus of infection, loweringchemoradiotherapy's side effect, prolonging live time and improving thequality of life. On account of various kinds of reasons, the research onmalignant bone tumor by TCM is relatively lagging, the knowledge on theregular pattern of the development of its TCM syndromes and itspathogenesis is still insufficient, the curative effect and mechanism ofTCM demands to be authenticated further by empirical study. On thesegrounds, this study made a clinical retrospective study on malignant bonetumor patients to explore its Traditional Chinese Medicine (TCM)syndromes and the characteristics of its TCM pathogenesis, and madeexperimental study on the mechanism of action that the method ofinvigorating vital energy and promoting blood flow and eliminating phlegmto treat malignant bone tumor.PartⅠThe study on the changes of TCM syndromesof malignant bone tumor patientsAim:This study tries to find out the changes of TCM syndromes and thecharacteristics of TCM pathogenesis of malignant bone tumor patients,and hopes to provide fundamental research for clinical applications and scientific research.Method: statistical analysis on correlated factors including type ofTCM syndromes of MM, age, gender, clinical stage, hemoglobinconcentration, Bence Jones protein etc. was carried out by means ofretrospective review.Results:There are very significant differences among different TCMsyndrome types(P＜0.01). In the MM patients, most TCM syndromes aredeficient syndrome(including qi deficiency and blood deficiency), bloodstasis syndrome, phlegm-dampness syndrome(including phlegm-turbiditysyndrome, damp-heat syndrome and cold-damp syndrome). The TCM syndromeshave no significant differences between MM patients above 60 years oldand under 60 years old(P＞0.05). The TCM syndromes have no significantdifferences between male or female MMpatients (P＞0.05). The TCM syndromeshave significant discrepancy between different clinical stages of MMpatients (P＜0.01). The TCM syndromes have significant discrepancy betweendifferent hemoglobin concentration MM patients (P＜0.01). The TCMsyndromes have significant discrepancy between different Bence Jonesprotein MM patients (P＜0.01).Conclusion: Deficient syndrome (including qi deficiency and blooddeficiency), blood stasis syndrome, phlegm-dampness syndrome are the mainTCM syndromes of MM patients. Phlegm, stagnant blood and deficiencyinteract and get more and more heavy effect during the process of MM'soccurrence and development. The TCM syndromes of MM patients have nothingto do with gender and age. There are close relationships between TCMsyndromes of MM and clinical stage, hemoglobin concentration, Bence Jonesprotein.PartⅡEmpirical study on herbs of invigorating vital energy andpromoting blood flow and eliminating phlegm treating tumor-bearing miceAim: To investigate the effect and mechanism of modified Decoction ofFour Drugs on the biological behavior of the in vivo infiltrating growthof malignant bone tumor.Methods:We build the mice orthotropic transplantation model ofosteogenic sarcoma KHOS. Observe the growth of the transplantation tumorafter treating with the modified Decoction of Four Drugs by intragastricadministration to interfere the biological behavior of the animal models; measure the expression of VEGF, KDR, Flt-1 of the transplantation tumorby the way of RT-PCR method.Results:high-dose modified Decoction of Four Drugs group hassignificant effect on restraining the proliferation of bone tumorcells(P＜0.01), while middle and low-dose groups have no significant effecton restraining the proliferation of bone tumor cells(P＞0.05),thetumor-inhibiting rate of high-dose group is higher than that of the middleand low-dose groups which are 3.98% and 9.24% separately. The high-dosemodified Decoction of Four Drugs group has the effect of significantlyinhibiting the expression of VEGF, KDR and Flt-1 of the transplantationtumor tissue, while the effect of inhibiting the expression of VEGF, KDRand Flt-1 of the transplantation tumor tissue by the middle and low-dosegroups is not significant; the effect of the high-dose Chinese medicinegroup on inhibiting the expression of VEGF, KDR and Flt-1 of thetransplantation tumor tissue is better than that of the middle andlow-dose groups(P＜0.05).Conclusion: High-dose modified Decoction of Four Drugs group canrestrain the growth of bone tumor in vivo in tumor-bearing mice. Theinvasion of bone tumor can be depressed by reducing the expression ofVEGF, KDR and Flt-1 to control the growth of new vessels of tumor in orderto interrupt the in vivo growth of malignant bone tumor.