The Experimental Study On Artificial Nerve Tissue Graft With Different Kind Of Material In Repairing Sciatic Nerve Defect In Rat

Objectives1. To investigate the histological characteristic of body wall of halobios Urechisunicinctus and the relationship between immunogenicity and decelularization processes ofchemically acellular body wall of halobios Urechis. To study the possibility of repairingperipheral nerve defect with artificial tissue nerve grafts composed of acellular body wallof Urechis unicinctus and medical absorption collagen foam rubber (Bodin), addingnerve growth factor(NGF).2. To study the possibility of repairing peripheral nerve defect with artificial tissuenerve grafts composed of acellular body wall of Urechis unicinctus and acellular nerveanografts, adding nerve growth factor(NGF).3. To investigate the possibility of repairing peripheral nerve defect with artificialnerve tissue grafts with surrounding fascia flaps and medical absorption collagen foamrubber (Bodin), adding nerve growth factor (NGF).MethodsA series of Sprawn Dawnley rats were randomly divided into 6 groups with 10 ratseach. The groups are Experiment Groupâ… , Experiment Groupâ…¡, Experiment Groupâ…¢,Auto-nerve Graft Group, Silione Tube Group and Normal Group.Experimental Groupâ… : A piece of acellular body wall of halobios Urechis was suturedinto a tube as nerve conduit. Within the conduit, medical absorption collagen foam rubber(Bodin) was filled to form an artificial nerve tissue grafts. Assisted with nerve growthfactor (Monosialotetraphenoxylganglioside) instillation, the sciatic nerve defect of 10mmlength was bridged with the artificial nerve tissue grafts.Experimental Groupâ…¡: Sciatic nerve defect of 10mm length in rat was bridged withthe artificial nerve tissue grafts composed of acellular body wall of Urechis unicinctusand acellular nerve anografts, adding nerve growth factor.Experimental Groupâ…¢: A piece of deep fascia was raised and sutured into a tubewith superficial surface as inner surface of the vascularized nerve conduit. Within theconduit, medical absorption collagen foam rubber (Bodin) was filled to form an artificialnerve tissue graft. Assisted with nerve growth factor instillation, the sciatic nerve defectof 10mm length was bridged.The Auto-nerve Graft Group and Silicone Tube Group: The sciatic nerve defects were bridge by auto-nerve graft and silicone tube respectively. Normal Group: Normalrats which no defects.The rats of all groups were breeded for 4 months postoperatively. At the end of thattime, electric physiologicl study, morphological and statistical study were performed.ResultAt end of 4 months, the function of sciatic neve was recovered well in the rats ofExperimental Groupâ… ,â…¡andâ…¢. The electric physiologicl study, morphological andstatistical study showed that the function of all the groups was superior to that ofSilicone Tube Group and Experimental Groupâ…¢approached the result of AutologousNerve Graft Group. The result of Experimental Groupâ…¢was superior to that ofExperimental Groupâ… andâ…¡.Conclusion1. To repair the peripheral nerve defect with artificial tissue nerve grafts composed ofacellular body wall of Urechis unicinctus and medical absorption collagen foam rubber,adding nerve growth factor is a feasible method. But the result is inferior to that ofautologous nerve grafts.2. To repair the peripheral nerve defect with artificial tissue nerve grafts composed ofacellular body wall of Urechis unicinctus and acellular nerve anografts, adding nervegrowth factor is a feasible method. But the result is inferior to that of autologous nervegrafts.3. To repair the peripheral nerve defect with tissue nerve grafts made of autologousfascial flap, medical absorption collagen foam rubber and nerve growth factor, whoseresult approachs that of autologous nerve grafts is a feasible method.