Primitial Explore The Polymorphisms Of Mitochondrial DNA And Ultrastructure Of Mitochondrion In Spleen Qi-deficiency Syndrome's Patient

Aim: To explore the whole genome sequence and ultrastructure ofmitochondrion in duodenum mucosa from spleen qi-deficiency syndrome's patient.To understand the relations between spleen qi-deficiency and polymorphismsof mitochondrial DNA (mtDNA).Method: Refer to having bordered the exclusive standard to choose caseswhich are divided into spleen qi-deficiency syndrome group,the wet and hotsyndrome group and normal contrastive group.By electron microscope to observethe ultrastructure of mitochondrial in duodenum mucosa. To extract andextend-prolifer mtDNA, to blast the result, to contrast with Cambridgesequence. Record the mutation Site,type,character and cipher code whichsynthesize amino acid. To understand the mtDNA's polymorphisms, make use ofOlige 6.0 software to analyse the multi-gene sequence.Result: 1 There are different degree's change in all groups. Nodifference is found between Spleen qi-deficiency syndrome and the wet and hotsyndrome group with same disease; there are obvious different between chronicshallow gastritis(CSG) and duodenum ulcer with same syndrome; CSG's changesare lighter and duodenum ulcer's changes are obviously more. Somemitochondrion swells, metamorphosis, which ridge become less, even vanish forduodenum ulcer of Spleen qi-deficiency syndrome, Some mitochondrion becomehollow; the wet and hot syndrome group's changes are like this, somemitochondrion get together. 2 The eleven samples are surveyed for the wholemtDNA sequence, the complete rate is 99%; every sequence is measured frompositive and negative two directions, some are several times, the result issteady. 3 There are 470 mutation after repeat measure. The mutation typeshave single and multi-nucleotide mutation, every mutation still includeexchange,insert and defect, there are 64 mutations in D-LOOP section, 53 mutations in t-RNA section, 77 mutations in r-RNA section, 276 mutations inCD section. The mutations are more in D-LOOP section from Spleenqi-deficiency syndrome and CSG. The defect mutations are more in duodenum ulcer.4 The alter code's mutations are 107, nucleotide exchange mutations are 314(same meaning mutations are 83, fault meaning mutations are 26, losing meaningmutations are 15), same meaning mutations,fault meaning mutations,losingmeaning mutations and alter code's mutations are less in Spleen qi-deficiencysyndrome than other's groups; fault meaning mutations and alter code'smutations are more in the wet and hot syndrome group than other's groups;same meaning mutations are more in CSG; fault meaning mutations,losingmeaning mutations and alter code's mutations are more in duodenum ulcer;transition and transversion are less in Spleen qi-deficiency syndrome group,insert and defect mutations are obviously more in duodenum ulcer group thanCSG group.Conclusion: 1 The ultrastructure of mitochondrion are different fordifferent disease(CSG and duodenum ulcer ) with same syndrome (Spleenqi-deficiency syndrome or the wet and hot syndrome group); 2 Spleenqi-deficiency syndrome's mtDNA exist polymorphism; 3 The mutations are morein D-LOOP section from Spleen qi-deficiency syndrome, which is probably itsmolecule base. 4 Insert and defect mutations are obviously more in duodenumulcer group of Spleen qi-deficiency syndrome or the wet and hot syndrome group,which is probably its molecule base.