| Irritable bowel syndrome (IBS) is a common chronic and non-organic intestinal disorder disease. Its main symptoms are recurrent abdominal pains, diarrhea, constipation or the alternating of diarrhea and constipation. Because of lack of evidence of pathologic and morphological change and abnormal biochemical index, it is included in IBS as FBD (Functional bowel disease, FBD). In the past, IBS was considered to be caused by disorders of bowel function and abnormal pains. However, in recent few years, many researches have demonstrated that IBS is related to the high sensitivity of the bowel. Now it is usually considered to be a heterogeneity disease with multiple causes caused by individuality.IBS is a common functional disease, nearly accounting for about 30%-50% of the gastrointestinal disease, of which the IBS with the main symptom of diarrhea is the most common among the patients. In recent years, a close relationship with psychological factors is reported both in domestic and international researches in this disease. Most patients have anxiety disorder,depressive illness or other mental and psychological abnormality, which may imply the failing of central nervous system' s adjustment to the intestinal neural system, the change involving hormone and receptors of partial muscle or nerve,the existence of the brain—bowel stalk and other factors. The western medicine nowadays prefers to use analgesics, antidiarrheal agents, antispastics and 5-HT3 receptor antagonist, drugs (such as loperamide and atropine) that relieve clinical symptoms,and drugs (such as oryzanolum, bifidobiogen-livzon, bifid Triple ciable capsule and dioctahedral smectite) that help regulate intestinal flora and vegetative nerve functional disturbance, or protect the bowel films. However, the long-term curative effect of these drugs is far from satisfactory, not to mention their side effects.The Traditional Chinese Medicine, however, has accumulated lots of experience in treating disease of intestines and stomach. There are also many useful formulas,which have little side effects,but better results. The value of herbs and formulas has gradually realized both at home and broad. In TCM, liver is thought to in charge of dipersing and regulation as well as qi activity. The emotional changes of the physical body are closely related to the flowing and circulation of qi and blood. Many researches have demonstrated that the function of the liver plays a critical role in our psychological reaction. The live is the core of the body in managing its psychological reaction. Animal experiments have showed that the herbs and formulas used for regulating the liver have some kind of effect in immune functions concerning the neurology and endocrine. In TCM, diarrhea is due to the spleen' s failing to transporting,which causes the stagnation of body fluid. Thus, functional disorders of the liver and spleen play a critical role during the process of IBS with diarrhea as the major symptom. The treatment from this point of view is also proved to be very effective. Formulas and treatments often used in clinic include the use of sanbai san, bupi yichang pill, yimucao decoction, jianpi tiaoqi tang, acupuncture, moxibustion, retention enema, injectio ad acumen, ear acupoint pressure therapy, acupuncture, massein and so on. Valid attempts have been made in the treatment of IBS, and also lots of experience has been accumulated. Besides, the new herb praeparatsion exploition is certain to have a broad prospect.The herb praeparatsion coming from the hospital is always considered to be the cradle of new drug, due to its credible clinical background. Many new drugs, such as fufang danshen drop pill, tongluo shenggu capsule, are all coming from in this way. Changyan ling tablet has been used as hospital clinical praeparatsion for 18 years in our hospital. The drug is mainly used in the treatment of diarrhea IBS, chronic colonitis and so on. However, because of lacking basic research, there are still many problems: our production craft falls behind, the standard for quality is not yet complete. That is why the drug is not effectively developed.Chang ji' an capsule is made by improving a traditional drug called changyan ling plan, which helps regulate spleen and liver, eliminate heat and wetness evil and antidarrhea with astringent. The capsule is constituted on the basis of sijunzi tang and tongxie yaofang: Dangshen, baizhu, fuling are used as principal drugs to invigorate spleen to excrete dampness; fangfeng, yanhusuo, muxiang, chenpi, baishao are the ministerial drugs for activating QI and invigoratsing spleen, together with huanglian, huotanmu, jiu biying, shiliu pi that can help eliminate damp and heat; gancao and baishao ease the affected liver to relieve pain. The whole formula invigorates the spleen and relives the liver,eliminate heat and damp and alleviates. Pre-experiment showed that the essential oil abstracted from the preparation produced effect probably by restraining excitability of histamine and rivalry bowels accentuation caused by neostigmine. Besides, it also has a counteract to Jimpy mice cramp pain reaction. This perfectly explains why it can alleviate patients' diarrhea and bellyache obviously. In conclusion , as a result of the animal experiment and the clinical research, the exploiture of the preparatsion is much promising.A mature and advanced technique for the speratsion and abstraction is necessary in the manufacture of a new valid preparatsion. SPE-CO is the most advanced physical technique currently. So we can make good use of it in the herbal manufacture. Its advantages such as superiority in extraction efficiency, shorter producing periods, complete valid constituent, less organic solvent residue, simple craft, smaller labor and less pollution. Therefore this technique is applicable to the craft for herbal abstraction.For the lack of animal models that are generally accepted now, the research of the disease is merely carried out in clinic. The models made by rectal injection with acetic acid is one of the methods to make animal model from outer stimulation. Theoretically speaking, the high sensitivity to ache is caused by continuate or repeated incitement of injury. During our clinical research, the IBS patients are usually found to have a gastrointestinal infection history, and often have irritable bowel syndrome during recovering periods. Generally speaking, thebaby rats' nerve structure is growing, and it is more sensitive and weaker. So it is much easier to have an effect on it. It represents: first, lack of available descending inhibitory system, second, the new-born spinal cord that gets excited much more easily. Therefore, if the animals are stimulated by some harmful injuries during these periods, their nervous tissue that is in charge of pains' transmission will get an allergy, even when they have grown up, and the former destructive stimulus has been eliminated for a long time, their intestine can remains to be highly sensitive. Viewing from this point, the chronic inflammation stimulus to baby rats, can cause a high bowel sensitivity in the adulthood. This tallies with the basic characters of IBS, and this method is worthy of further tries.The herbal products used for the treatment of intestine disease include pills, dispersers, pieces, capsules, oral liquid and so on, most of which are based on traditional crafts, including water distilling, wine distilling, vapor distilling of naphtha, heating, evaporatsing, concentratsing, cyclic sirocco drying and so on. But much valid composition gets lost during the producing procedure, especially the part that is sensitive to heat. Besides, the quality and effect are not stable at all. That is why most valid herbal preparatsions are not completely empoldered and grow into a industry, their application limits in doctors' prescription. With the help of modern technique, it is an inevitable trend to empolder and develop the traditional herb, and new preparation, and new craft and stable standard are necessary anyway.In the program, We adopt the SFE-CO2 technique to extract active ingredient from the compound prescription herbs, with the experiment result of pharmacy and curative efficacy as the evaluation index. According to the requirement of the new herbal preparatsion, the research and evaluation are merely carried out on the medicine whose efficacy has already been testified in clinic. We also adopt many new techniques, such as GC-MS fingerprint,HPLC, TLC etc, in order to survey and control the craft parameter. So the pre-clinical research of the new medicine must be safe, valid, stable and also kept in control. Only in this way, could we make sure of the stability, reduce the taken dose, lower the side effect, and explore the mechanism finally. A strict principle in the program is quite significant for the research of the mechanism about chang ji' an capsule.Along with the quicker modern living rhythm, the change of the life style and the food structure, it seems a trend that the incidence of IBS rises year by year, but not for the use of a patent medicine of the same kind at the same time. Chang ji' an capsule treats IBS with the diarrhea as the main symptom, according to different clinical performance, combining with the etiological factors and pathogenesis, to treat IBS from the liver and spleen, and the formula is quite reasonable, Although it only takes a very short time, the clinical background is quite credible, and the foreground of the new preparatsion is vast both in the domestic and international markets, and it is quite worthy of being made good use of. Section I A Study of the Craft of Chang ji' an capsule production . Part I Chang ji' an capsule medicine material source and examination. Ob]eete: To check the source and quality of the raw material medicine. Methods:Char appreciation, powder micro-appreciation, thin-layer chromatography (TCL) appreciation, extract, total ash, moisture content, acid-indissoluble ash, pesticide residue determination and high performance of liquid chromatogram(HPLC) are used to determine the content of the aim ingredient.Results:each medical material char, appreciation,check,content determination, etc are all consistent with the requirement.Conclusion: According to The Pharmacopoeia of China 2005 relevant specifications, the medicine material's quality corresponds to the regulation, so it can be follow-up studied.Partâ…¡The grading-up of supercritical fluid extraction's condition.Objective: To grade up supercritical fluid extraction specification's condition, of baizhu, fangfeng, muxiang, chenpi.Methods: According to the influencing factor of SFE-CO2 abstraction, select the three factors: pressure,temperature,time, and every factor has three levels, use L9(34) orthogonal table to improve the craft condition of extraction, and take it by two reproducibility experiments, i.e.: Take about 308g of the coarse powder of baizhu, fangfeng, muxiang, chenpi, group it in the HA221-50-06 SFE-CO2 (1000ml) equipment, accommodate the temperature to 45°C, and extract pressure at 25Mpa. Resolution pressure is at 6Mpa, and resolution temperature is 45°C, the flow rates of CO2 is 21.7L/h, then carry out 3 hours. Then gains the abstractive product land 2, weigh the product, determine costunolide and dehydro-α-curcumene. Calculate the extraction percentage of costunolide and dehydro-α-curcumene.Results:The analysis of range and analysis of variance show that: the factors influencing extraction and costunolide dehydro-α-curcumene volume dose are:(A) extractive pressure >(B) extractive time >(C) extractive temperature. Among of them (A) and (C) are main factors, which possess the statistics meaning. So the best combination is A3B1C3: pressure is 26Mpa,time 3h, temperature is 45°C.Conclusion: SFE-CO2 average yield of the prescription is 3.72%. The volume dose of costunolide and dehydro-α-curcumene is 995mg. Extraction percentage is 95.5%. The result of the two experiments is the same. It shows that the craft of SFE-CO2 is stable and the repeated experiment can be made.Partâ…¢The grading-up of water extraction condition.Objective: To select the best water extraction craft technique condition.Methods: According to the design of craft route, the remnant of baizhu, fangfeng, muxiang, chenpi left after the extraction of the SFE-CO2, together with dangsheng, baishao, shiliupi, jiubiying, gancao, fulin, and the three factors water extraction,extraction time and frequency. Every factor has three levels. The experiment is run in L9 (34) orthogonal layout. The index is total weight of solid substance, content of peoniflorin. To investigate the best parameter, the best condition experiment will be made twice.Results: From analysis of range and analysis of variance, it can be seen that the influencing factor of total solid substance and peoniflorin content can be presumed as C (extract frequency) >A (water duty) >B (extract time), effect of C and A are the principal having statistical significance. The best combination is A3B1C3. Due to the small effect of B (extract time), in order to save time and energy source, we choose B2. So the best combination is decided to be A3B2C3, I.e.: 800% water, extract 3 times, each time lasted 1h.Conclusion: The total solid substance average dose of baishao and other medicine by water extraction is 138g, peoniflorin total amount is 2121mg, extraction percentage of peoniflorin via water extract is 88%. The results of tow repeat experiments are the same. It explains that the craft is stable and repeated experiment can be made.Partâ…£The choice and optimization of drying craft.Objective: With peoniflorin as the index constituent, to screen the craft of drying spray,vacuum dehydration and normal pressure drying, and to grade up drying condition.Methods: Calculate drying product weight from the three drying craft and determinate the content of peoniflorin, then calculate peoniflorin total changing rate, repeating these experiments, and then decide drying method. Using drying condition as effect factor to physic liquor blowing character,adhesive wall phenomenon,drying time, calculate the remaining rates and total changing rates, and decide the drying parameter.Results: The result of the experiments proves that if heating too long, peoniflorin would be lost seriously when normal pressure drying and vacuum dehydration is used; drying spray has feasibility, time is short, production is clean, loss of peoniflorin is light, so it is better than other methods. The repeated experiment result shows that, weight of dry powder is 367g, compare with the paste before drying spray, remaining rate is 79.5%. Compared with medical material, the changing rates of peoniflorin is 70.0%. The results of two experiments are the same. It explains that the craft is stable and the repeated experiment can be made. So we will choose drying spray for this craft. The parameter results: the temperature of inlet is 180°C, the temperature of outlet is 100°C, air exhausting parameter is 95%, pump parameter is 25%. The result of the repeated experiment indicates: dry Dower of water extract paste is 366g, peoniflorin weight is 1609g, compared with paste before drying spray, changing rates is 75.82% compare with medical material, total changing rates is 66.08%. The two results of the repeated experiments are the same.Conclusion: The drying spray craft and drying parameter stabilization and the repeat experiment can be made.Partâ…¤Grading-up the craft parameter of preparing granules.Objective:Grading-up the craft parameter of preparing granule.Methods: Take dry power of prescription dose add yanhusuo,huanglian fine powder and supercritical fluid extraction, misce bene, and choose different parameters when granulate, granulation is 20-40 mesh. Observe the granules characters.Results: The result indicates that corpuscular firmness,fine grits proportion are close related to feed material speed of granulator,roller rotation speed and granulate speed.Conclusion: The best granulate parameters are: 48rpm of feed material speed, 30rpm of roller rotation speed and 30rpm of granulate speed.Sectionâ…¡Establishment of the quality standards of Changji' an CapsulePartâ… Identify by TLCObjective: To build up a TLC method to identify yanhusuo, huanglian jiubiying, shiliupi, huotanmu, baizhu in Changji' an Capsule.Methods:Extract the contents in the capsule by organic solvent, filter, condense and determine the volume, to manufacture solution for the article aqua. Also the differentiate nicety to check against the control article, and add organic solvent to dissolve, metered volume, as control article solution. With the prescription not containing target material, press the praeparatsum craft and use the trial article aqua preparative method, as negative sample solution. Three samples are respectively in the thin layer plate of the same gel silica G, with agent spread out, examine under the ultraviolet light, the visible light responding to lamellar spots may be inspected.Results:The reference substance and the contents in the capsule display the same lamellar spot in the corresponding position in the colour spectrum, while the negative control does not have any interference..Conclusion: The methods to identify the yanhusuo, huanglian, jiubuying, shiliupi, huotanmu, baizhu in the capsule are stable can be used to identify Changji' an Capsule.Partâ…¡The limit check on arsenic salt, heavy metal, moisture content, disintegration time limit, microbial limit. Objective: To check the arsenic salt, heavy metal, humidity, disintegratsion time, microbial limit of three batches of Changji' an Capsule.Methods: Follow the methods containing in Provisions for New Drugs Approval and Chinese Pharmacopeia I edition, 2005.Results:The content of arsenic salt is less than 2 ppm, the content of heavy metal is less than 10 PPM, the moisture content is 6.73%, disintegration time limit is 4.5 min. No large angstrom sievert bacterium is checked; bacterial number,mold,yeast count,coli group are all<10/g.Conclusion: The arsenic salt, heavy metal, humidity, disintegratsion time are all consistent with the regulation of Chinese pharmacopeia I 2005 edition. The microbial limit is consistent with the microbial limit standards of praeparatsum for oral use.Partâ…¢Determining of the characteristic compositionsobjective: To determine the content of peoniflorin in baishao, costunolide and dehydro-α-curcumene in aucklandiae, and make up a reasonable content limit.Methods:Column: Phenomenex luna C18(250mm×4.60mm, 5μm);mobile phase: acetonitrile-water, gradient elution; flow rates:0.8mL·min-1; column temperatsure:30℃; detecting wavelength 230 nm; sample size:10μl. The number of theoretical plate can not be less than 2000 according to peoniflorin, and can not be less than 3000 according to costunolide and dehydro-α-curcumene.Results:The linear relationship is fine if the content of peoniflorin is between 0.1516-3.032 ug, costunolide is between 0.1116-2.232 ug, dehydro-α-curcumene is between 0.1014-2.028 ug, coefficient correlation r=0.9999; the results of density test,stability test,repeatability test: each peak area score's RSD are less than 2%,the recovery rate of peoniflorin is 100.90%, RSD=1.75%;the recovery rate of costunolide is 101.12%, RSD=1.80%; the recovery rate of dehydro-α-curcumene is 101.37%, RSD=1.22%。the result of sample detection: peoniflorin((?)±s): 3.38±0.05mg, RSD(%):1.43, costunolide ((?)±s): 1.66±0.03mg, RSD(%):1.82, dehydro-α-curcumene ((?)±s): 1.87±0.02mg, RSD(%): 1.05.Conclusion:Three kinds of characteristic compositions are detected by the same method of HPLC with good precision, repeatability and stability. According to the results and the minimum content limit of Chinese pharmacopeiaâ… , the content of peoniflorin(C23H28011) in baishao could not be less than 1.6%,each capsule contain 0.105g, according to the prescribed drugs, if the transfer rate is 40%, this article content is determined as: Each capsule contains peoniflorin no less than 1.25 mg, accounted with the peoniflorin (C23H28011);According to the measurement result, each capsule contains costunolide and dehydro-α-curcumene of volume cannot be less than 1.8% at the level with the lowest limit content and muxiang. According to the prescription(each capsule contains muxiang 0.042g), then the limit content is: each capsule contains costunolide (C15H2002) and dehydro-α-curcumene (C15H1802) can not be less than 1.05 mg, in 40% transfer-rate. Partâ…£The GC- MS fingerprint of SFE-CO2 extraction in the Changji' an CapsuleObjective: To build up the GC- MS finger print of SFE-CO2 extraction in Changji' an Capsule for controlling the praeparatsum's internal quality.Methods: The SFE-CO2 extraction, supersound extracting, analysis the finger print by GC- MS, combining the computer index technique to identify the structure of the separated chemical compound, and the relative contents in percentage were calculated with Area Normalization Method.Results: Each composition in Changji' an Caps SFE-CO2 extraction are all well separated. There are 13 compositions be separated, 10 compounds structure are identified.Conclusion: The GC- MS method can be used to establish finger print of the volatil ingredients in Changji' an Caps.Sectionâ…¢The experiment of the main pharmacodynamics of Changji' an capsule.Objective:According to the main pharmacodynamics of changji' an capsule, to study its function of regulate spleen and liver, eliminate heat and wetness evil and antidarrhea with astringentMethods: Checking diarrhea experiment, small intestines' propulsion experiment, anti-inflammatory experiment, and relieving pain experiment.Results: This Chinese medicine (which is called changji' an capsule) can prolong incubation period of defecating dark stool and reduce egesta of small rats, and have a certain function of checking diarrhea, reduce the small intestine peristalsis speed, lower the permeability of blood capillary, and achieve an anti-inflammatory effect. It can also prolong the incubation period of the experiment small rats' body stretching; reduce the frequency of body stretching, and have analgesic effect especially on the ache caused by chemistry substance.Conclusion: Changji' an capsule has certain functions of checking diarrhea, anti-inflammatory and analgesic effect, and provides the pharmacodynamic basis for treating Diarrhea-IBS, chronic colitis, chronic indigestion, stomachache, diarrhea, etc.Sectionâ…£Establishment of rats experiment model with Diarrhea-IBS and the valuation of its internal organs sensitivityObjective:To build up the rats experiment model with Diarrhea-IBS, observe the times of the rats defecation and the value threshold change of ectocolon and the influence of bowel movement by adding ACH.Methods:Give the acetic acid to stimulate the rectum of the rats new-born only for 8 days everyday, keep on for 2 weeks, duplicate the model of high sensitivity of internal organs of rats and count the number of egesta of model rats on 14th days, 15th days, 16th days within 4 hours. Evaluate threshold value of rats' belly shrink reflect (AWR) and 6 weeks and 8 weeks after they were born., they have their rectum extended. Measure the rising range of the bowl taken from the model rat movement on which ACH is affected.Results: Compared with the normal groups, the model groups show defecation times increasingly, and its egesta are not confectioning molding. When the rectum be extended, the press use threshold value and belly raising value of the model group are cut down more obviously than those of the normal groups. After ACH is joined into the isolated bowel, the value of the model group goes up, all obviously higher than those of the normal groups.Conclusion: Stimulating the bowel of model rats in new-born period by the chronic inflammation can cause chronic bacillary diarrhea and increase the internal organs sensitivity in adult, and increase the reaction of the spasm agent which stimulate the isolated bowel, all those essentially match Diarrhea-IBS basic characteristics.Sectionâ…¤Research on the Mechanism of Changji' an Capsule Intervening Diarrhea-IBS Model Rats.Objective: To study the intervention mechanism which Changji' an capsule has on Diarrhea-IBS model rat.Methods: Randomly divided rats of new-born into normal group, model group, Chinese herbal medicine in high quantity group, Chinese herbal medicine medium quantity group, Chinese herbal medicine low quantity group and the pill of Bupi yichang pill group. New -born rats in normal group are given 0.9% physiology in rectum, the other groups from 8th day to 21st day are given the acetic acid to stimulate the rectum everyday, keeping on 2 weeks, duplicating the model of high sensitivity of internal organs of rats and counting the number of egesta of model rats on 14th days, 15th days, 16th days within 4 hours. After 8 weeks, infuse the stomach to give respectively the NS, Changji' an capsule liquid 3.76g/ kg weight, 1.88g/kg weight, 0.94g/kg weight and Bupi yichang pill 6.76g/kg weight, once daily, for two weeks, observe the rats again after medicine is taken and evaluate its belly constringency reflect threshold (AWR) value. Measure the rat blood with the cell instrument in a percentage for distribution of T lymph-cell, observes the rat bowel organization of immunity dyeing of 5-HT. Observe those cell degranulation of mast cell and count mast cells number with light microscope, and observe MC cells and their adjacent structures.Results:Changji' an capsule can raise the press threshold value of sensitivity of the model rats, and has diarrhea checking function to Diarrhea-IBS. It can increase CD4+ value in blood gradually, CD8+ and the specific value of the CD4+ CD8+ which recover normally, improve the immunity function. Changji' an can lower increasing secretion 5-HT of the model rats of IBS, improve allergy of internal organ, diarrhea symptom and motive abnormality caused by 5-HT going up unusually, in each group of colon glue surface and no ulcer or debauched was seen, the gland body size and appearances are normal, the glandular organ mucus and cup cell form were seen abnormal, a little amount cell of phlegmasia inside the quality is gradually changed, each colon organization has no change, matching the clinical pathology of IBS performance. The experiments show that the Chinese herbal medical has certain function on the IBS chronic bacillary diarrhea and phlegmasia diseases. The number of mast cells of proper layer and degranulation in model group is higher than those of normal group obviously, and the number of mast cell of proper layer and degranulation in high quantity group is smaller than those of model group, but is not difference with the normal group. It shows that, the medicine liquid can reduce the mast cell quantity, improve the model rats colon organization in the mast cell degranulation, and the instauratsion that make it normal. The medium quantity group also can improve degranulation of mast cells obviously, showing it has the function of stabilizer, this function may be one of main mechanism that changji' an capsule treating IBS, gives an electric mirror, to observe mast cells to locate the bowel to proper layer, implying many grains with higher electronics density in the afterbirth syrup; The mast cells of model group has part of degranulation, empty bubble formation; Each group can be seen to have mast cells and the nerve fibers are adjacent. As a result, it expresses that a MC possibility that activates the appearance is in the IBS expression with the motive change, the felling abnormality for characteristic of internal organs raise the decisive function in the pathologic physiology process of the high sensitivity. Conclusion: The function mechanisms of Changji' an capsule intervening Diarrhea-IBS are: (1) Improve sensitivity of bowel of the model rats of IBS; (2) Reduce times of loose stools;(3)Improve the cell immunity function of the model rats; (4) Improve bowel abnormality caused by the unusual increase of 5-HT and allergic feeling of internal organs; (5) Relieve intestinal mucosa edema and inflammatoryreaction caused by IBS. (6) Reduce the mast cell quantity, improve the colon organization degranulation, have the function that stabilizes mast cells. The result for further demonstrating the disease occurence mechanism of IBS and taking intervention measures helps establish necessary research foundation. This shows that the overall function of multiple targets of Chinese herbal medicine in its intervention of IBS. Section VI Peculiarities and New ideas in this Study.Firstly, The IBS is a common chronic disease with characteristic of high incidence rate, long course and slow recovery. This research which exploited hospital praeparatum with exact curative effect, aims at the disease characteristics, makes traditional prescription and modern medicine techniques combine together, It adopts advanced techniques of SFE- CO2, spraying dry technique of fog etc., excellent craft, and better exertive efficacy of medicine, It reduces stimulus and makes use of the modern and analytical technique of GC- MS, HPLC etc. to control its quality strictly, establishes the volatility composition fingerprint diagram, and makes the craft to have the advanced novelty. Secondly, currently there are many animal related models of IBS, but built up according to the single factor outside the body that mostly matches the IBS animal model on some pathologic physiology mechanism, but the combination of disease and symptoms in models is not reported. Feeling mood factor has something to do with symptom of IBS when it occurs and aggravates, and feeling mood can cause liver gloomy. This experiment chooses to use the model stimulated by the chemistry chronic inflammation, and the incitement placed in the new born period of new born rats, belonging to the animal model which caused by earlier period event, so it is also a kind of feeling mood factor to the rats. Chronic inflammatory stimulation in bowel in new born rats may cause the chronic internal organs sensitivity to increase after they become adult, matching IBS basic characteristics. Thus, building up of the model rats with Diarrhea-IBS have stability and repetitions advantages, the maneuverability is strong, it has characteristic of being evaluated, thus having important and realistic meaning to the further sieving of the treatment medicine. Finally, most researchers of the world concentrate upon clinic study of IBS. there are few studies based on animal experiment. This article studies in detail the mechanism of Changji' an capsule from sensitivity of bowel, 5-HT, mast cell, mast.cell degranulation etc, thus enriching the contents of IBS basic research, and explaining complex action of multitargets.
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