Row Of Terminal Differentiation Of Erythroid Cell Nuclear Mechanism And Differentiation Related Gene Cloning And Identification

Developmental biology has been the hot point of biological sciences over a century since it was founded by German biologist Roux and his colleagues in 1894. Among so many active fields of developmental biology, cell differentiation and dedifferentiation has being paid more and more attention. When cells become committed to particular developmental fates, they undergo a series of divisions and differentiations, and finally give rise to terminally differentiatal cells specialized in certain functions. If this program is disturbed by some unexpected factors, differentiations are hindered, and cells even become tumor cells. Under some enviromental conditions, some tumor cells redifferentiate and give rise to normal functional terminal cells. So cell differentiation-dedifferentiation-redifferentiation is a dynamic process, and the investigation of its mechanism can provide us some important dates for tumor and its therapy researches. Terminal differentiation of mammalian erythroblasts is characterized by two main peculiar features, such as the active expression of globin gene, the pyknosis and denucleation to the anucleus rcticulocytes. Erythroblasts could become erythroleukemia cells and lost the above characteristics of differentiation stage when the differentiation process is blocked. And under certain conditions, erythroleukemia cells might regain its differentiation capacity and undergo normal differentiation. So erythroblasts provide a good model for the study of cell differentiation and dedifferentiation.Up to now, the reports on the erythroblast differentiation regulatory factors are mainly focused on the early stage of erythroblast, the early erythroblasts are known to be the last stage of EPO dependant. The factors that may regulate the further processes of differentiation and maturation are not known. A series of characterized changes such as cell division stopping, nuclear pyknosis and denucleation, however, are not certain and need to be elucitated. During recent years, Dr. Xue's group has made intensive studies on the factors related to the terminal differentiation of mammalian erythoblast, including the process of denucleation . Their early works are mainly focused on the morphological...