| Food safty, is not only related to the people's livelihood, is also an important symbol thenational civilized degree. Foodborne pathogens is the most important risk factor and hasbecome a global public safety problem. While in the food safty researching, the resistant genein the food chain transfer is often ignored. Beacaus of the extensive use of antibiotic in themedical and the animal husbandry, some antibiotics leaked into the environment with medicalwaste and animal feces, and after diffuse which became subinhibitory concentrations ofantibiotics. The origin of resistance gene in the food chain transfer is in environmentalmicrobial. However, how does the resistance gene transfer under the stimulation sub-inhibitconcentration of antibiotics of transmission is an impact mechanism which was not wellunderstandood. So identifying the potential risk of resistance gene transimited into humanwas restricted. In this study was from the point of view the food safety problems, andfoodborne pathogen was used as the object. First, the rapid detection of drug-resistant genemethods was established, and these were used to test food samples in common resistance genedistribution. Then the effect of resistance genes transfer were tested under the stimulation ofsubinhibitory concentrations of antibiotics. And the mechanism of resistance gene horizontaltransmission was discussed from the protein expression level and RNA transcription level.Finally the research findings of the molecular mechanisms of drug resistance gene wereutilized to inhibit the horizontal gene transmission. When TesB protein expression wasincreased, no matter the presence of low dose ciprofloxacin stimulation, spread of drugresistance gene can be effectively inhibited (P <0.01). The understanding of esistance genehorizontal transmission can be increased by findings of the study. A feasible researchdirections was provide to inhibit drug resistance genes transfer in the food chain transfer.
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