Diabetes Based On Nano-biological Systems, Multi-parameter Detection System

Diabetes is a familiar disease and its incidence is high in China and all over the world. The incidence of diabetes in developmented country is above 6%-10% and in China is about 2%-4%. Diabetes is attracted high attention of world, and the number of diabetes sufferer increased gradually in recent years.There are many complications of diabetes. These complications could cause many bad results if they could not be treated effectively. How to detect and inspect diabetes effectively is a very important question all the while. This doctor paper is a part of Chinese 863 item. It detect and inspect diabetes through enzyme biosersor and can detect four parameters synchronously. The biosensor we designed used carbon paste as electrodes, and enzymes were immobilized on the surface of electrodes through special bio-immobile technology. It could detect four parameters which are related to diabetes and its incidence, the four parameters are blood glucose, total cholesterol, lactic acid and p-hydroburyrate. We discussed detailedly about how to design the biosensor and how to confirm their working parameters in this paper. We accomplish the designing and experimental work about the biosensor and finish all the clinical experiments with satisfactory results.The biosensor we designed is a microanalysis sensor based on carbon paste electrodes, it is very special. After analyzing the electrode process and characteristic of kinetics and doing research on reaction mechanism of enzyme, we construct the kinetics model of the system. The i-t transient model accords well with our experiments.We introduce carbon nanotubes into biosensor to improve the capability of biosenor. We found mat linear range of glucose biosensor was increased after add carbon nanotubes into the glucose oxidase solution. We construct the theoretical model to explain this result in macroscopical and microcosmic angles.