Cobalt-60gamma, Ray Irradiation Of Mutagenic Effect And Mechanism Of The Daffodils

By using the Chinese narcissus bulb as material, the study had been focused on the ⁶⁰Co(?)-rayirradiation-induced effects and mechanism in cytology and molecular biology The study wascomposed of five parts: 1.changes of morphology 2.cytological effects 3.changes of physiologyand biochemistry 4.DNA polymorphism and phylogenetic analysis among different dosage by⁶⁰Co(?)-ray 5. The changes of compose and content of flower essential oil by GC/MS.In November in Hefei area i.g. corona-crown differentiation stage of Chinese narcissus, injuryrates of nutrient organs were higher and higher with the dosage of ⁶⁰Co(?) -ray increments. Itwas displayed dominantly in 1. length of leaves shorten, which was only 40% of controlmaterial, 2. height of scapes decreased distinctively, which was only about 30% of control, 3.the number and length of roots decreased too, which difference of morphological charactersamong three dose rates (4.4Gy/min, 8.7Gy/min, 12.6Gy/min) was insignificant. After flowerbud differentiation was finished, the injury rates of morphological characters of nutrient organsand reproductive organs were lower than that in corona-crown differentiation. However, theradiation sensitivity of Chinese narcissus in various differentiation stages was different. In theearly stage it was higher than that in the later stage, There were power function relationship(Y=aX^b) between the length of leaves, the height of scapes and radiation dosages. They wereInY=3.6779-0.264llnX and lnY=4.0872- 0.9430lnX respectively And the correlationcoefficient was 0.9538 and 0.9672 respectively There were line function relationship (Y=a+bX) and quadratic function between the damage rate of leaves and height of scapes andradiation dosages. They were Y=-2.l6l6+l.560X and Y=-l2.456+3.l467X-0.03279X²respectively The correlation coefficient was 0.9762 and 0.9844 respectively TO obtain bigvariety the differentiation stage should be selected at the early stage and the radiation dosageshould be more than l5Gy.Gamma ray irradiation induced aberration of M1 root tip cell nucleus and chromosomalaberration. There were abundant aberrant types radiated by⁶⁰Co(?)-ray They were chromosomebridge, chromosome fragment, laggard, free chromosome, chromosome pulverizaion,chromosome disjunction and nuclear bleb, nuclear disruption, nuclear fragmentation,micronucleus (which contains single micronucleus, double micronucleus, multi-micronucleus),nuclear ear nuclear fission, nuclear budding. Line correlation between the rates of differentchromosomal aberration and radiation dosages existed. ABSTRACTThe total rate of chromosomal aberraion was respectively 4．52ï¼…,14．旧％,17．43ï¼…,54．56ï¼…,75石0ï¼…,96．85ï¼…with SGy3 10Gy3 15Gy3 25Gy9 35Gy3 50Gy radated by60CQY－p TheresutSof ANOWshowed that there was highly significant difference between control materials and 60＿＿。＿】。,＿＿＿＿．】．．＿r＿卜t＿】＿＿＿＿＿＿＿:＿＿＿＿L＿．,＿J'L＿。L．＿LiatCfi3I F＆diatCd bV一Lny,fgu Afl口 thC fCSllltS OI ill＊plC COffipatlSOfl SflOWCQ tflat llgllsignificant existed between hheh dosop and low dosape,but if the difference between wodoså‘›es was only SGXthe dlffefCnce of chfomosomal aberration Me was not obvlo鹏．叮临rate of cell nuclear劝enå¹»ion was similar to that of chromosomal aberation．They wererespectively 5．38ï¼…,10,12ï¼…,16．27ï¼…,23．04ï¼…,38．84ï¼…,sl,23ï¼…with SGyt 10Gy3 15Gy3 25Gy3。－。。－。。t。,,60n＿＿＿＿＿＿＿＿＿＿＿＿rLL＿J三＊〕口二＿＿＿'＿＿＿＿三'！－上。＿上二＿＿J二＿。:＿＿'L＿35口V sfld 50GV mdfdluCd bV""LO'y－fapbCCallSC Ott＊eæ—¥lflCfCttt SCflsltlVIV OI fry＆QlatlO几 TllCdifference ofcbomosomal劝erratlon nd nuclear aberå¹»ion between corona－crown dlvlslon ＿＿……．。,。,60＿·,'·。＿】＿＿'】and after flower bud dlvlslon was lmportam striKing．frs刚lation of一乙w－r叫lrmaollea celldw...