Nifa Protein Function And Multiple Copies Of Nifa Gene Promotion, Nitrogen Fixation Efficacy Of S. Meliloti (sinorhizobium Meliloti,) | | Posted on:2004-04-29 | Degree:Doctor | Type:Dissertation | | Country:China | Candidate:C T Yang | Full Text:PDF | | GTID:1113360122471029 | Subject:Genetics | | Abstract/Summary: |  PDF Full Text Request | | Replacement of the conserved amino acid residue Thr-290 by Val in the C3 central domain region of Klebsiella pneumoniae (Kp) NifA resulted in a loss of transcriptional activation of nif genes. Thus, the conserved Thr-290 residue appears to be critical for functional activation of the NifA central domain. We used this point mutant in a merodiploid experiment to examine the putative multimerization function of the NifA central domain. The result of this experiment showed that this domain contained the multimerizartion determinants of the NifA protein. A series of truncation mutants of NifA was constructed to determine those structural elements within the central domain that are critical for multimerization. Our data demonstrate that amino acid residues 252-453 are involved in the multimerization process. | | Keywords/Search Tags: | Sinorhizobium meliloti, Enterobacter cloacae, NifA protein | | PDF Full Text Request | Related items |
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