Effect Of ERK1/2 And PI₃K Signal Pathway For Human Endometrium Of Abnormal Uterine Bleeding Caused By IUD Through The Treatment Of Regulating Liver To Stop Bleeding

Purpose:Investigate the mechanisms of adjusting liver to stop bleeding to cure the uterus abnormal bleeding side-effect with IUD, in order to provide new thought and method to cure haemorrhagia, and to provide experimental foundation for further investigation of the basic theory of TCM that liver storing blood.Method:Primary culture the Endometrial tissue, then treat them with Drug-containing serum, use western blot and ELISA method to check the leve of Phosphorylation of ERK1/2 and PI3 K signal pathway; Acquire endothelial cell of human spiral arteries by homogenization and enzyme digestion. Then observe the effect of the serum containing Chaihu Zhixueye by testing NO,ET that the endothial cell excrete.Results:â‘ The patients of No.1,3,5 belong to liver can not store blood pattern, they have abnormal uterine bleeding, for No.l, Chaihu zhixueye-containing serum have a tendency of increasing the level of phosphorylation of ERK1/2, Guipitang-containing serum have a tendency of increasing the level of phosphorylation of PI3K; for No.3, Chaihu zhixueye-containing serum have a tendency of increasing The level of phosphorylation of ERK1/2; for No.5, Chaihu zhixueye-containing serum have a tendency of increasing the level of phosphorylation of ERK1/2 and PI3K.â‘¡The patients of No.4,8,9 belong to spleen deficiency pattern, they have little amount of abnormal uterine bleeding, Guipitang-containing serum have a tendency of increasing the level of phosphorylation of ERK1/2 and PI3K.â‘¢The patients of No.2,7 belong to both liver depression and spleen deficiency pattern, Guipitang-containing serum have a tendency of increasing the level of phosphorylation of ERKl/2, and Danzhixiaoyaosan-containing serum have a tendency of increasing the level of phosphorylation of PI3K; The patient of No.6 belongs to both liver depression and spleen deficiency pattern, she does not have abnormal uterine bleeding, Danzhixiaoyaosan-containing serum have a tendency of increasing the level of phosphorylation of ERKl/2.â‘£For the 2 patients in induced abortion group, compare to orther serum, serum of middle dosage of Chaihu Zhixueye can maintain the the ratio of NO/ET.Conclusion:In order to treat Abnormal uterine bleeding after using IUD, Chaihuzhixueye may have the mechanism of regulating the level of phosphorylation of ERK1/2 to advance vasoconstriction and vascular repair, platelet aggregation, activate coagulation of uterine tissue of liver excessive patients, and also may regulating the level of phosphorylation of PI3K to advance the effect of cell proliferation and inflammatory. Chaihu Zhixueye can adjust the activity that endothelial cell excrete ET and NO. So that endothelial cell can accelerate repair of uterus spiral arteries vessels then stop the abnormal bleeding.