Functions Of Hsa-miR-145 On Prostate Cancer PC-3 Cell Lines

Part One:Functions of hsa-miR-145 on prostate cancer PC-3 cell linesObjective:To investigate the functions of human miR-145 mimics en prostate cancer PC-3 cell lines.Methods:By up-regulation of the cellular concentration of activating miR-145 mimics, we observed and detected the alternation of biological functions of PC-3 cell lines. To investigate the cell proliferation ability by MTT assay, colony formation assay and EdU assay. To understand the cell apotosis by AnnexinV/FITC-PI stained flowcytometry. To calculate the cell cycle phases chaged by PI stained flowcytometry. At last, we measured the ability of invasiveness and metastasis by cell adhesion assay, wound healing assay and transwell assay.Results:Our experiments demonstrated that over-expression can inhibit the proliferation of PC-3 cells(MTT assay, P<0.05:colony formation assay; EdU assay, P<0.05), promote their apotosis(P<0.05); arrest the cell cycle at Go/Gl phase(P<0.05) and decrease their ability of invasiveness and metastasis.(P<0.05).Conclusion:miR-145 mimics can inhibit the proliferation of PC-3 cells, promote their apotosis, arrest their cell cycle and decrease their cell invasiveness and metastasis. It acts as a tumor suppressor gene and may be a potential therapeutic target for the low expression cancers. Part Two:Meta-AnalysisLymphatic Sparing Versus Lymphatic Non-Sparing Laparoscopic Varicocelectomy in Children and Adolescents:A Systematic Review and Meta-AnalysisObjective:The aim of this study was to explore the true efficacy and potential advantages of lymphatic sparing laparoscopic varicocelectomy (LSV) over lymphatic non-sparing laparoscopic varicocelectomy (LNSV) in children and adolescents.Methods:A search was made for studies published up until 31 August 2010 using the terms "lymphatic sparing varicocelectomy", "laparoscopy", "adolescent" and "children" in the Medline, Embase, Ovid, Web of Science, and Cochrane databases. Randomized controlled trials (RCTs) and observational clinical studies (OCSs) comparing LSV and LNSV were included. A systematic review and meta-analysis was erformed using odds ratios (ORs) for dichotomous variables.Results:Out of 127 studies,2 RCTs and 4 OCSs were eligible for inclusion, comprising 489 cases of LSV and 307 cases of LNSV. The hydrocele rates after LSV were signifi cantly lower than after LNSV (OR=0.19; 95%confi dence interval [CI]=0.10 to 0.36; p < 0.00001). However, no significant difference was observed between LSV and LNSV with regard to recurrence (OR=0.65; 95%CI=0.32-1.33; p=0.24) or catch-up growth (OR =2.14; 95% CI=0.97-4.72; p=0.06).Conclusion:LSV is valuable in reducing the incidence of postoperative hydrocele, with similar recurrence and catch-up growth rates as LNSV. A series of RCTs are warranted to explore the efficacy of LSV in the management of varicocele in children and adolescents.