The Clinical And Experimental Research Of Sanqi Combined With Tamoxifen On Breast Cancer

ObjectiveSanqi (panax notoginseng) and tamoxifen are used in combination to breast cancer patients. Explore the influence of this treatment to liver injury and gynecologic events through regular monitoring of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase and applying B-ultrasound to evaluate the change of fatty liver and ovarian and uterine events. Provide a new clinical thought to endocrine therapy of breast cancer.Method68 patients of breast cancer were randomly divided into two groups. The group of treatment included 34 cases:taking moxifen+SanQiPian, continuously 12 months; The group of tamoxifen included 34 cases either:taking tamoxifen for 12 months. Observation items:①Monitor liver function:Every three months evaluate glutamic oxaloacetic transaminase and glutamic pyruvic transaminase once;②Evaluate fatty liver degree and ovarian and uterine events with B-ultrasound every three months;③During th period of treatment,will evaluate the treatment safety including serious cardiovascular events, venous thrombosis events and metastasis and tumor recurrence.ResultPart 1 The effect of liver function and fatty liver:After the treatment of 6,12 months, the glutamic oxaloacetic transaminase and the glutamic pyruvic transaminase increased significantly, the treated group and the control group all got before-and-after significant difference(P<0.05). The treated group increased significantly lower than the control group there was statistics difference between two groups. After 12 months, The number of abnormal liver function increased significantly than before treatment in two groups,but there was no statistics difference between two groups. With the extension of treatment,the number of Mild and moderate fatty liver increased, especially in control group, After 12 months, the number of fatty liver in treated group increased lower than control group. there was statistics difference between two groups.Part 2:The main influence of tamoxifen to premenopausal women is increasing the opportunity of hysteromyoma.The control group gots before-and-after significant difference(P<0.05). To postmenopausal women the main influence of tamoxifen was endometrial proliferous. The treated group and control group all gots before-and-after significant difference(P<0.05).Assessment of treatment safety:There were not any cardiovascular events and venous thromboembolic events, and other serious adverse reaction during the treatment. Two groups of patients during treatment were not local recurrence and distant metastasis. ConclusionSanqi (panax notoginseng) and tamoxifen combined application of breast cancer treatment in blood stasis resistance, can effectively protect liver function, reduce the fatty liver happen opportunity; can slow premenopausal women with tamoxifen treatment related uterine fibroids generation and increases; to postmenopausal women can protect endometrium, reduce endometrial unusually proliferous, thus improve treatment adherence. The treatment effect of tamoxifen was not affected by the combined group during the treatment. Combination therapy has no serious adverse reactions and has good safety. ObjectiveTo study the effects of panaxnotoginseng spaonins(PNS) and tamoxifen (TAM) on growth and apoptosis of breast cancer cell line MCF-7 and T47-D which express different ERβ. Explore whether the combination can increase the effects of anti-cancer and find possible mechanism. Evaluate the influence of ERβto treatment.MethodPNS and TAM were used individually or in combination in different concentration to MCF-7 and T47D cell. Cell proliferation was evaluated by MTT assay.Distribution of cell cycle and rate of apoptosis were determined by flow cytometry.ResultBoth TAM and PNS could inhibit the growth of MCF-7 and T47D cells, The effects of inhibition depend on concentration and time. TAM(1.5×105mol/L)combined with three different concentration of PNS or was used individually showed remarkable advantage to other groups; Compared with the untreated group, both TAM and PNS could increased the quantity of MCF-7 cells in G0/G1 phase and reduced the quantity of T47D cells in S phase. The two drugs combination showed remarkable advantage in apoptosis than individual drug. Under the same conditions, individual TAM and PNS showed obvious advantage on growth and apoptosis of breast cancer cell line MCF-7 and T47-D of ERβpositive than ERβnegative. The two drugs combination got the same result.ConclusionPNS showed the direct effects of inhibition to breast cancer cell line MCF-7 and T47D cell, which depended on concentration and time. High concentration of combination of two drugs could increase the effect of inhibition of MCF-7 and T47D cells,. The mechanisms related with the influence of cell cycle and promote apoptosis. The combination of two drugs could increase the effects of anti-cancer. PNS and TAM were used individually or in combination showed obvious advantage on growth and apoptosis of breast cancer cell line MCF-7 and T47-D of ERβpositive than ERβnegative.The influence of of cell cycle were different between different combination. The mechanisms maybe related with the influence of cell cycle and promote apoptosis.