Non-barbiturate Intravenous Anesthetic Agents On Rat Cortical Astrocytes Express Thrombospondin 1 And Neuron Number Of Synapses In Rats

Objective:To investigate the modulation of TSP-1 levels in astrocytes by etomidate, midazolam and ketamine and observe the influence on synapses number of co-cultured neurons by etomidate and ketamine.Method:Cortical astrocytes were cultured then treated with etomidate, midazolam and ketamine at different concentrations. The Sprague-Dawley rat was used as the primary cell source. The TSP-1 expression was detected through RT-PCR and Western blotting. We established co-culture model of neurons and cortical astrocytes and observed the numbers of synapses of co-culture neurons after being treated with etomidate and ketamine.Results:1) Etomidate promote the expression of TSP-1.2) Ketamine reduce the secretion of TSP-1, though there is no significant difference compared with the control group.3) Midazolam has no notable effect on the modulation of TSP-1.4) Etomidate increases the number of synapses of co-cultured neurons while ketamine reduces it.Conclusion:1) Etomidate promote the expression of TSP-1.2) Ketamine reduce the secretion of TSP-1.3) Midazolam has no obvious effect on the modulation of TSP-1. 4) Etomidate and ketamine has different influence on the synapses number of neurons.