| [Object] Spinal cord injury is a common and frequent diseases, mainly due to movement, high-altitude falls, traffic accidents, injuries and other causes. Spinal cord injury is a traumatic disease, and will bring the patients whole life suffering. The patients will become paralyzed because of losing a part or all of the motor and sensory, unable to work and take care of themselves. It will be a great burden to the family and society. Currently, the incidence of spinal cord injury grows up constantly with the social development. So the research work of spinal cord injury recovery becomes a hot issue in the world. The main research fields include following aspects:First, transplantation of cells and spinal cord to the damaged spinal cord tissue to repair the spinal cord injury, such as olfactory ensheathing cells, schwann cells, neural stem cells, umbilical cord blood stem cells, and fetal spinal cord tissue. Second, Research on the genetic level:by inhibiting the expression of apoptosis gene in order to inhibit the apoptosis of nerve cells and protect the damaged spinal cord tissue. Third, the use of some bioactive agents such as nerve growth factors, hormones and other drugs which can promote nerve growth and regeneration, as well as protect the affected nerve tissue. Fourth,To reduce spinal cord injury scar formation. Fifth, the use of omental transplantation to restore and rebuild the blood supply of the damaged spinal cord tissue, and promote its regeneration. Although various methods above may play a role in the treatment of spinal cord injury, but we still have a long way to go to fully respire the damaged spinal cord tissue and restore the original motor and sensory functions. So the subject of spinal cord injury research is still the hot issue in the world. In this experiment, after the treatment of autologous platelet-rich plasma (PRP), by the techniques of pathological section, evoked potentials, inclined plane test, BBB scale, immunohistochemistry, computer graphics, and western-blot, the recovery of neurological function, the nerve fibers growth through the glial scar and the nerve growth factor NGF, BDNF expression were observed.[Methods] Male SD rats were divided randomly into PRP treatment group (group A) and the control group (group B).2ml blood was collected retroorbitally, and by the modified Appel method, autologous PRP (group A) and plasma (group B) was prepared. Both groups used intraperitoneal anesthesia with ketamine hydrochloride lOOmg/kg+xylazine hydrochloride 25mg/kg. Modified Nystrom method was used to establish the experimental animal model of acute spinal cord compression injury in posterior thoracic segment. The damaged spinal cord tissue was instilled with autologous PRP (group A) or plasma (group B). After the operations, the following studies were conducted:①inclined plane test,②BBB motor function,③HE staining observation and the remaining area of spinal cord tissue,④somatosensory evoked potential (SSEP),⑤two types of nerve axons calcitonin gene-related peptide (CGRP)-positive and 5-HT nerve fibers in the situation across the glial scar detected by double immunofluorescence technique,⑥neurotrophic factors NGF, BDNF expression changes detected by western-blot technique.[Results]①In the 7th and 14th day after spinal cord injury, the hindlimb motor function score of group A was significantly higher than that of group B (P<0.05). SSEP latency and amplitude values in the 14th day after spinal cord injury:12.37±1.689ms and 0.54±0.056mv in group A,14.14±1.553ms and 0.32±0.03lmv in group B, differences between the two groups was statistically significant (P<0.05).②In the 14th day after spinal cord injury, the remaining area of spinal cord tissue of group A was larger than that of group B (P<0.05). The axonal sprouting in group A was significantly better than group B (for 5-HT axons:40.2±4.98%vs 28.3±3.22%, for CGRP-positive axons:58.3±4.23%vs 43.2±3.26%) (P<0.05). In group A, there is significant difference between 5-HT group and CGRP group (P<0.05), while in group B, there is no significant difference between 5-HT group and CGRP group (P> 0.05).③In the 3rd,7th and 14th day after spinal cord injury, the neurotrophic factors NGF, BDNF protein expression showed an upward trend in both group A and B, In the 7th and 14th day after spinal cord injury, The neurotrophic factors NGF, BDNF protein expression of group A was higher than that of group B (P<0.05).[Conclusion] Autologous platelet-rich plasma instillation in the damaged area of the spinal cord can significantly improve the repair of the injury spinal cord.
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