Screening Of Differentially Experssed Proteins In Pancreatic Cancer And Protein Expressed In Pancreatic Cancer With Kras Mutation

Purpose:Pancreatic cancer is the fourth leading death of cancer among men and women in the United States The overall survival rate is less than 1% at 5 years with most patients dying within one year[9]. The lack of diagnosis early method and effective treatment is the main problem to pancreatic cancer. We aim to identify protein signatures of pancreatic cancer for diagnosis and therapy.Materials and Method:we employed protein pathway array method to assess the protein expression of 11 paires pancreatic cancer samples,5 pancreatic samples and 2 normal pancreatic samples. Next, we use high resolution melting (HRM) curve analysis for KRAS mutation of all pancreatic cancer samples. In addition, Pathways associated with these expression changes were identified using the Ingenuity Pathway Analysis tool.Results and Conclusion:8 proteins were upregulated and 4 proteins were downregulated in pancreatic cancer compared with and normal tissues. KRAS mutations were identified in 9 of 15 tumor tissues (60%). p-PKCα/βⅡin K-ras positive upregulation. diffirent express protein mainly affects cell cycle, cellar Growth and Proliferation, Tissue Morphology network functions.IPA result demonstrated the proteins of our study is correlation with lots of signaling pathway.Eight proteins (FAS, cdk2, cdc2 p34, EGFR, Akt, Vimentin, E-cadherin ICAM-1) were up-regulated and four protein (cyclin B1, Cdc25B, H-Ras, HIF-3α,) were down-regulated in pancreatic cancer compared with normal tissues.these differentially expressed proteins affect cell proliferation, cell cycle and apoptosis ect.these proteins can be used to early diagnosis and therapeutic target.