Effects Sarcandra Glaba Having On PI3K/Akt/mTOR Signal Pathway Of Human DU-145 Prostatic Cancer Cells

Objectives:To observe the efficacy Sarcandra Glaba having on the multiplication of human DU-145 prostatic cancer cells in hormone-independent prostatic cancer as well as the principle of it restraining DU-145 cells to multiply.Methods:Cell cultivation was adopted with different groups using 2.5mg/ml,1.25mg/ml and 0.625mg/ml Sarcandra Glaba concentrations on DU-145 cells for 48 hours each. The DU-145 cells'morphology was observed under optic microscope. The multiplication of DU-145 cells was measured by MTT method. Both ELLISA and RT-PCR methods was used to measure the concentrations and expressions of PI3K, Akt, mTOR and P70S6K.Results:DU-145 cells'morphologic changes were shown with number decreasing, volume reduction, chromatin coagulation and nucleus contraction after 48 hours'influence Sarcandra Glaba concentrations had on them. A certain quantity-effect relation was shown in each concentration group. The values of OD of all PI3K, Akt, mTOR and P70S6K were lowed by the concentration groups measured with ELLISA. The expressions of all PI3K, Akt, mTOR and P70S6K were lowed by the concentration groups measured with RT-PCR. The two methods of measurement showed the same results.Conclusions:Sarcandra Glaba concentrations can gave enough damage to the normal growth of DU-145 cells as well as restrain it to multiply. The principles of these effects can be related on the restraining effects of the concentrations and expressions of all PI3K, Akt, mTOR and P70S6K. The 4 factors above are regarded as the most important factors of PI3K/Akt/mTOR signal pathway which plays a critical role in the occurrence and development of hormone-independent prostatic cancer.